|Title||Characterization of Cervidpoxvirus isolates from Oregon, California, and eastern Canada. |
|Publication Type||Journal Article |
|Year of Publication||2009 |
|Authors||Moerdyk-Schauwecker, M, Eide, K, Bildfell, RJ, Baker, RJ, Black, W, Graham, D, Thompson, K, Crawshaw, G, Rohrmann, GF, Jin, L |
|Journal||Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc |
|Date Published||2009 Jul |
|Keywords||Virus Cultivation |
The present report describes the analysis of 4 Deerpox virus isolates from California, Oregon, and Ontario, Canada. All 4 isolates were associated with cutaneous crusting lesions. Examination of selected samples by electron microscopy demonstrated that the viruses were morphologically similar to orthopoxviruses. Phylogenetic analysis of the A21 gene, which is found in all poxviruses, indicated that the 4 isolates form a lineage distinct from other members except for those belonging to the genus Cervidpoxvirus of the subfamily Chordopoxvirinae. Members of the Cervidpoxvirus lineage encode a set of genes not found in other poxviruses. These include homologs of genes encoding interleukin 1 receptor antagonists (IL-1Ra) and C-type lectin-like receptors (CTLR). In the current investigation, genes encoding homologs of IL-1Ra and CTLR were amplified from all the isolates and were found to be closely related to orthologs found in the Cervidpoxvirus genus, which further supports the inclusion of these isolates in the Cervidpoxvirus genus.