TitleChloroform-free permeabilization for improved detection of β-galactosidase activity in Vibrio cholerae.
Publication TypeJournal Article
Year of Publication2017
AuthorsToulouse, C, Häse, CC, Steuber, J
JournalJ Microbiol Methods
Volume137
Pagination1-2
Date Published2017 Jun
ISSN1872-8359
KeywordsBacterial Proteins, beta-Galactosidase, Cell Membrane, Chloroform, Detergents, Genes, Reporter, Lac Operon, Nitrophenols, Promoter Regions, Genetic, Protease Inhibitors, Transcription, Genetic, Vibrio cholerae
Abstract

LacZ (β-galactosidase) is used to monitor the transcription of genes in reporter strains carrying the lacZ gene under the control of a promotor of interest. This protocol for LacZ activity determinations in Vibrio cholerae following detergent lysis results in 2.5-fold increase of LacZ activities compared to lysis with chloroform.

DOI10.1016/j.mimet.2017.03.011
Alternate JournalJ. Microbiol. Methods
PubMed ID28336461