Test Name: Diagnostic Section: Fee:
Avian Paramyxovirus - virulent
Newcastle Disease PCR
Molecular Diagnostics
Species: Set up days: Turn Around Time:
Poultry and Wild Birds  M, Tu, W, Th, F  2-3 days
Specimen Requirements:    

Specimen of Choice: oropharyngeal/tracheal swab (pool up to 5 birds per tube)
Tissues: lung, trachea, spleen, brain (individually bag each tissue, keep each bird separate)

Collection Protocol:    
Aseptically obtained specimen(s); place swabs in sterile tube with 2-3 ml of BHI broth or viral transport media.  Viral transport media obtained from VDL-Virology may be used.  Submit each tissue individually bagged and labeled with permanent marker. 
Shipping Requirements:    
Refrigerate swabs, freeze tissues, leakproof container(s).  Overnight shipping recommended
Additional Information:    
Do not use bacterial transport media or culturettes containing agar or charcoal because they may have an inhibitory effect on the PCR assay.  *NAHLN assay*