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BVDV-PI PCR - pooled ear notches
|Test Name:||Diagnostic Section:||Fee:|
|BVDV-PI PCR - pooled ear notches||Molecular Diagnostics
|Species:||Set up days:||Turn Around Time:|
|Bovine||M, Tu, W, Th, F||2-3 days
|Single ear notch (1cm/side) place in a dry, sterile tube (e.g., red-top serum tube- no separator gel or anticoagulant). No Ziploc or whirl-pak bags. Do not send large pieces of ear tissue. (see information below for recommended ear notch tool)|
Collect ear notch (1cm/side) from each animal and place in a dry, sterile tube (e.g., serum tube-no separator gel or anticoagulant) 6 ml with push up plastic cap preferred. Individual samples must be identified and submitted separately; samples will be pooled by the laboratory. Up to 24 ear notches/pool (no pooling if <10 samples submitted - please refer to individual BVDV-PCR or BVD ELISA test information).
A medium ear notch tool works well (e.g., Nasco: 800-558-9595, size-Medium C0024N, or Valley Vet Supply: 800-419-9524, size-Medium 17016, or similar tool that cuts a notch that is ½” wide at base).
|Refrigerated, leak proof containers. Ship overnight Monday-Thursday only.
|Fee is $55.00/pool. Individual specimens within a positive pool will be identified by BVDV ELISA for an additional fee of $3.50/ear notch. Samples from different owners will not be pooled. Pooled testing should only be used for detection of Persistently Infected (PI) animals in herds without clinical signs of BVDV. Although it is possible to detect acute BVDV infections, clinical suspects should be tested individually to avoid false negative results. If an animal tests positive, we recommend testing again in 3-4 weeks to confirm PI status.|