BVDV-PI PCR - pooled ear notches

Test Name: Diagnostic Section: Fee:
BVDV-PI PCR - pooled ear notches Molecular Diagnostics
$45.00
Species: Set up days: Turn Around Time:
Bovine M, Tu, W, Th, F Average: 1-2 days
Specimen Requirements:

Single ear notch (1cm/side) place in a dry, sterile tube (e.g., red-top serum tube- no separator gel or anticoagulant).  No Ziploc or whirl-pak bags. Do not send large pieces of ear tissue. (see information below for recommended ear notch tool)
Collection Protocol:

Collect ear notch (1cm/side) from each animal and place in a dry, sterile tube (e.g., serum tube-no separator gel or anticoagulant) 6 ml with push up plastic cap preferred. Individual samples must be identified and submitted separately; samples will be pooled by the laboratory. Up to 24 ear notches/pool (no pooling if <15 samples submitted - please refer to individual BVDV-PCR or BVD ELISA test information.

Disinfect tools with 10% bleach (e.g., 100ml (3oz) bleach in 900ml (27oz) water) or 1% Virkon; rinse thoroughly with water between animals. Specimens MUST be free of dirt, feces, tattoo ink and vaccine. Residual disinfectant or contamination with feces or tattoo ink can yield false negative results. Do not vaccinate while collecting ear notches - this can lead to contamination with extraneous BVDV, causing false positive results.

A medium ear notch tool works well (e.g., Nasco: 800-558-9595, size-Medium C0024N, or Valley Vet Supply: 800-419-9524, size-Medium 17016, or similar tool that cuts a notch that is ½” wide at base).

Keep refrigerated for a maximum of 72 hours, DO NOT FREEZE.
Not for testing animals exhibiting clinical signs - please refer to individual BVDV-PCR test information.
Shipping Requirements:

Refrigerated, leak proof containers. Overnight shipping recommended.
Additional Information:

Fee is $45.00/pool. Individual specimens within a positive pool will be identified by BVDV ELISA for an additional fee of $3.00/ear notch. Samples from different owners will not be pooled. Pooled testing should only be used for detection of Persistently Infected (PI) animals in herds without clinical signs of BVDV.  Although it is possible to detect acute BVDV infections, clinical suspects should be tested individually to avoid false negative results. If an animal tests positive, we recommend testing again in 3-4 weeks to confirm PI status.