Equine Herpesvirus 1/EHV-1 PCR

Test Name: Diagnostic Section: Fee:
Equine Herpesvirus Type 1/EHV-1 neuropathogenic and wild type PCR Molecular Diagnostics  $48.50 *
Species: Set up days: Turn Around Time:
Equine  M, Tu, W, Th, F  2-3 days
24 hours if **STAT
Specimen Requirements:    
EDTA whole blood, deep nasal swab. Recommend testing both nasal swab and blood. Will test both specimens from the same horse for a single *fee. 
Collection Protocol:    
Collect whole blood in EDTA tube, collect nasal swabs (synthetic swab, plastic shaft) in sterile tubes (no anticoagulant or gel).    Refrigerate samples.  Do not allow blood to freeze.
Shipping Requirements:    
Refrigerated, leak proof containers.  Overnight shipping with ice packs.
Additional Information:    
This Allelic Discrimination PCR assay will differentiate between wild type and neuropathogenic genotypes.  *Fee is $48.50 each (includes testing of both nasal swab and blood from the same horse) for the first 2 animals; each additional animal at the same time from the same owner is $33.50 per test.
**STAT fee will be added

 

PCR Testing of non-symptomatic, exposed horses is NOT recommended

Submit serum samples for testing non-symptomatic horse for exposure to the EHV-1 virus

 

  • Request EHV-1 serology

 

Suspect EHV or EHM case (live horse with clinical disease).

  • Wear disposable gloves and change gloves between each horse
  • Collect whole blood into EDTA and label sample (preferably have an assistant label samples)
  • If a twitch is used to restrain the horse, it must be washed and disinfected between horses
  • Nasal swab collected using Dacron tipped swab with plastic shaft. Swab should be in contact
  • with nasal mucosa for at least several seconds.
  • Swabs are available from the VeterinaryDiagnostic Laboratory. Please call 541-737-3261.
  • Place swab in a sterile tube, (no anticoagulants or gel) and label sample.
  • Perform hand hygiene between horses sampled and put on new pair of examination gloves
  • Request the EHV-1 real-time PCR test and virus isolation (additional feel for virus isolation).
  • The real-time PCR test performed by the OSU VDL provides typing of the virus based on the
    DNA polymerase gene, distinguishing the neurotropic marker from the non-neurotropic marker
  • Keep samples cool but not frozen and ship by overnight delivery to the following address:

Veterinary Diagnostic Laboratory
Oregon State University
134 Magruder Hall
Corvallis, OR 97331-4801