Equine Herpesvirus 1/EHV-1 PCR

Test Name: Diagnostic Section: Fee:
Equine Herpesvirus Type 1/EHV-1 neuropathogenic and wild type PCR Molecular Diagnostics  $50.00 **
Species: Set up days: Turn Around Time:
Equine  M, Tu, W, Th, F  2-3 days
24 hours if STAT*
Specimen Requirements:    
EDTA whole blood, deep nasal swab. Recommend testing both nasal swab and blood. Will test both specimens from the same horse for a single fee. 
Collection Protocol:    
Collect whole blood in EDTA tube, collect nasal swabs (synthetic swab, plastic shaft) in sterile tubes (no anticoagulant or gel).    Refrigerate samples.  Do not allow blood to freeze.
Shipping Requirements:    
Refrigerated, leak proof containers.  Overnight shipping with ice packs.
Additional Information:    

This Allelic Discrimination PCR assay will differentiate between wild type and neuropathogenic genotypes. Also referred to as the N752 or A2254 genotype (non-neuropathogenic allele) and the D752 or G2254  genotype (neuropathogenic allele).

Perkins, et al./Veterinary Microbiology 139 (2009) 375-378

*STAT fee will be added
**Fee is $50.00 each (includes testing of both nasal swab and blood from the same horse) for the first 3 animals. If 4 or more tests are submitted from the same herd at the same time all tests are $42.50 each.

PCR Testing of non-symptomatic, exposed horses is NOT recommended

Submit serum samples for testing non-symptomatic horse for exposure to the EHV-1 virus

  • Request EHV-1 serology

Suspect EHV or EHM case (live horse with clinical disease).

  • Wear disposable gloves and change gloves between each horse
  • Collect whole blood into EDTA and label sample (preferably have an assistant label samples)
  • If a twitch is used to restrain the horse, it must be washed and disinfected between horses
  • Nasal swab collected using Dacron tipped swab with plastic shaft. Swab should be in contact
  • with nasal mucosa for at least several seconds.
  • Swabs are available from the VeterinaryDiagnostic Laboratory. Please call 541-737-3261.
  • Place swab in a sterile tube, (no anticoagulants or gel) and label sample.
  • Perform hand hygiene between horses sampled and put on new pair of examination gloves
  • Request the EHV-1 real-time PCR test and virus isolation (additional fee for virus isolation).
  • The real-time PCR test performed by the OSU VDL provides typing of the virus based on the
    DNA polymerase gene, distinguishing the neurotropic marker from the non-neurotropic marker
  • Keep samples cool but not frozen and ship by overnight delivery to the following address:

Veterinary Diagnostic Laboratory
Oregon State University
134 Magruder Hall
Corvallis, OR 97331-4801