Equine Herpesvirus real-time PCR Panel

Test Name: Diagnostic Section:


Equine Herpesvirus real-time PCR Panel Molecular Diagnostics  $50.00 *
Species: Set up days: Turn Around Time:
Equine M, Tu, W, Th, F  1-3 Days - average
**24 hours if STAT
**Same day,  if STAT and specimen is  received before 10:00 am
Specimen Requirements:    
Nasal swab, EDTA whole blood.
Collection Protocol:    
Collect nasal swabs (flocked swab or Dacron tipped swab with plastic or wire shaft) in dry sterile tubes without anticoagulant or gel; plain red-top tube works well. Flocked swabs are preferred.  Collect whole blood in sterile EDTA tube - mix well.   Refrigerate samples.  Do not allow blood to freeze. See specimen collection recommendations below.
Shipping Requirements:    
Refrigerated, leak proof containers.  Overnight shipping with ice packs.
Additional Information:    

The EHV real-time PCR Panel includes screening for EHV-1 and EHV-4 viruses. If EHV-1 DNA is detected, the specimen will be further tested using the EHV-1 allelic discrimination real-time PCR assay. The EHV-1 allelic discrimination PCR assay will differentiate between wild type (non-neurotropic) and the neurotropic genotypes, also referred to as the N752 or A2254 genotype (non-neuropathogenic allele) and the D752 or G2254 genotype (neuropathogenic allele).  Perkins, et al./Veterinary Microbiology 139 (2009) 375-378  

*Fee is $50.00 per specimen for the first 3 specimens and then $42.50 each for additional specimens submitted at the same time from the same owner.

**STAT fee will be added for STAT requests.

  • PCR Testing of non-symptomatic, exposed horses is NOT recommended
  • Submit serum samples for testing non-symptomatic horse for exposure to the EHV-1 virus and request EHV-1 and EHV-4 serology

Specimen collection from suspect EHV or EHM case (live horse with clinical disease):

  • Wear disposable gloves and change gloves between each horse
  • Collect whole blood into EDTA and label sample
  • If a twitch is used to restrain the horse, it must be washed and disinfected between horses
  • Collect nasal swab using a flocked swab or Dacron tipped swab with plastic or wire shaft
  • Swab should be in contact with nasal mucosa for at least several seconds
  • Place swab in a sterile tube, (no anticoagulants or gel) and label sample
  • Swabs are available from the Veterinary Diagnostic Laboratory. Please call 541-737-3261