Test Name: Diagnostic Section: Fee:
Equine Respiratory Disease PCR Panel - PCR Panel (EHV-1, EHV-4, Influenza Type A and EVA) Molecular Diagnostics $120.00*
Species: Set up days: Turn Around Time:
Equine M, Tu, W, Th, F 1-3 Days - average
24 hours if STAT** (specimen must be received by 10am)
Specimen Requirements:    

Nasal swab and EDTA whole blood

Due to variability in viral shedding, it is recommended that both nasal swab and EDTA whole blood be submitted for detection of EHV. Blood is tested for EHV only. Nasal swab must be submitted for detection of EHV, Inf-A and EVA by PCR.

Collection Protocol:    

Collect nasal swabs (flocked swab or Dacron tipped swab with plastic or wire shaft) in dry sterile tubes without anticoagulant or gel; plain red-top tube works well. Flocked swabs are preferred. 

Collect whole blood in sterile EDTA tube - mix well.   Refrigerate samples.  Do not allow blood to freeze.

Shipping Requirements:    
Refrigerated, leak proof container. Overnight shipping with ice packs.
Additional Information:    

The EHV real-time PCR Panel includes screening for EHV-1 and EHV-4 viruses. If EHV-1 DNA is detected, the specimen will be further tested using the EHV-1 allelic discrimination real-time PCR assay (no additional fee). The EHV-1 allelic discrimination PCR assay will differentiate between wild type (non-neurotropic) and the neurotropic genotypes, also referred to as the N752 or A2254 genotype (non-neuropathogenic allele) and the D752 or G2254 genotype (neuropathogenic allele).  Perkins, et al./Veterinary Microbiology 139 (2009) 375-378  

*$120 fee includes testing on both a nasal swab and EDTA blood. Testing a nasal swab only is $70.00 for the first 3 specimens and then $42.50 each for additional specimens submitted at the same time from the same owner. For a blood specimen the fee is $50.00 is tested for EHV only.

**STAT fee of $25 will be added for STAT requests.