Diagnostic Section: 
Molecular Diagnostics
Shipping Requirements: 
Ship overnight in an insulated container, with ice packs.
Test Fee: 
Setup Days: 
Turn Around Time: 
1-3 days, Same day if STAT** (specimens must be received by 10am)
Specimen Requirements: 

Serum, blood-swab, EDTA blood, lung, spleen, lymph node or tonsil tissue

Collection Protocol: 

Collect 1-5 g tissue or at least 0.5 ml serum (removed from clot) into sterile containers. If using a serum separator tube, please centrifuge prior to shipment.

For blood-swab, completely soak swab with fresh blood and place into a sterile tube (break off shaft) containing several drops of sterile saline or PBS. Just enough to cover the swab tip, not the shaft. A sufficient amount of blood can be obtained by using a 20 gauge needle to nick an ear vein, or while ear notching young pigs. 

To prevent PCR inhibition:

Use synthetic swabs (Dacron or Polyester) with a plastic or wire shaft. 

DO NOT USE COTTON SWABS (NO Q-TIPS) or swabs with wooden or paper shafts. Do not use swabs that contain gel or calcium alginate.

Additional Information: 

This real-time RT-PCR assay detects both the European (EU) and North American (NA) subtypes of PRRSV. Results for each subtype are reported.

**STAT fee of $30 will be added for STAT requests.