Test Name: Diagnostic Section: Fee:
White Nose Syndrome real-time PCR
(Pseudogymnoascus destructans)
Molecular Diagnostics $34.00*
Species: Set up days: Turn Around Time:
Bat M, Tu, W, Th, F 2-4 days
Specimen Requirements:    
 Skin swab, environmental swab.
Collection Protocol:    
Dip the tip of a dry sterile swab into a sample tube (2 ml microcentrifuge tube) containing approx. 150 ul sterile nuclease-free water to moisten the tip (most water will be absorbed by the swab).

Sample one of the bat's forearms and adjacent wing tissue between the elbow and wrist by gently ROLLING the swab across the surface of skin (three passes back & forth). Rolling the swab as it is moved along the skin prevents abrading the delicate wing skin.

Place the swab tip into the same sample tube used to moisten the swab, and break off the protruding plastic shaft of the swab without touching the rim of the tube or inside of lid with your fingers. Close the lid of tube tightly.

Do not use bacterial transport swabs or any tubes containing agar gel or charcoal, as these are inhibitory for PCR. Hold swab samples chilled (4°C) if they are to be shipped within 2 days following collection; otherwise freeze samples at -20°C until they are shipped. If stored at -20°C, samples should be received by the lab within 4 weeks from collection.
Shipping Requirements:    
 Refrigerated, leak proof containers.  Overnight shipping recommended
Additional Information:    
* Fee is $34 each for the first 4 tests or $29 each if 5 or more samples are submitted at the same time - same owner - per accession.