Anaerobic transformation of 2,4,6-TNT by bovine ruminal microbes.

TitleAnaerobic transformation of 2,4,6-TNT by bovine ruminal microbes.
Publication TypeJournal Article
Year of Publication2004
AuthorsFleischmann TJ, Walker KC, Spain JC, Hughes JB, Craig AM
JournalBiochemical and biophysical research communications
Volume314
Issue4
Pagination957-63
Date Published2004 Feb 20
ISSN0006-291X
KeywordsAnaerobiosis, Animals, Biodegradation, Environmental, Cattle, Chromatography, High Pressure Liquid, Rumen, Spectrophotometry, Ultraviolet, Trinitrotoluene
Abstract

Degradation of TNT by bovine rumen fluid, a novel source of anaerobic microbes, was investigated. Whole rumen fluid contents were spiked with TNT and incubated for a 24h time period. Supernatant samples taken at 0, 1, 2, 4, and 24h were analyzed by reverse-phase HPLC with diode array detection. Within 1h, TNT was not detectable and reduction products of TNT including 2-hydroxyl-amino-4,6-dinitrotoluene, 4-hydroxylamino-2,6-dinitrotoluene, and 4-amino-2,6-dinitrotoluene were present with smaller amounts of diamino-nitrotoluenes. Within 2h, only the diamino and dihydroxyamino-nitrotoluene products remained. After 4h, 2,4-diamino-6-nitrotoluene and 2,4-dihydroxyamino-6-nitrotoluene were the only known molecular species left. At 24h known UV absorbing metabolites were no longer detected, suggesting further transformation such as complete reduction to triaminotoluene or destruction of the aromatic ring of TNT may have occurred. TNT was not transformed at 24h in autoclaved and buffered controls. This study presents the first direct evidence of biodegradation of TNT by ruminal microbes.

Alternate JournalBiochem. Biophys. Res. Commun.