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Analysis of the genome of leporid herpesvirus 4.
|Title||Analysis of the genome of leporid herpesvirus 4.|
|Publication Type||Journal Article|
|Year of Publication||2012|
|Authors||Babra B, Watson G, Xu W, Jeffrey BM, Xu J-R, Rockey DD, Rohrmann GF, Jin L|
|Date Published||2012 Nov 10|
|Keywords||Animals, Base Composition, Cells, Cultured, DNA, Viral, Genome Size, Genome, Viral, High-Throughput Nucleotide Sequencing, Humans, Inverted Repeat Sequences, Molecular Sequence Data, Open Reading Frames, Phylogeny, Rabbits, Restriction Mapping, Sequence Analysis, DNA, Simplexvirus, Viral Proteins|
The genome of a herpesvirus highly pathogenic to rabbits, leporid herpesvirus 4 (LHV-4), was analyzed using high-throughput DNA sequencing technology and primer walking. The assembled DNA sequences were further verified by restriction endonuclease digestion and Southern blot analyses. The total length of the LHV-4 genome was determined to be about 124 kb. Genes encoded in the LHV-4 genome are most closely related to herpesvirus of the Simplexvirus genus, including human herpesviruses (HHV-1 and HHV-2), monkey herpesviruses including cercopithicine (CeHV-2 and CeHV-16), macacine (McHV-1), bovine herpesvirus 2 (BHV-2), and a lineage of wallaby (macropodid) herpesviruses (MaHV-1 and -2). Similar to other simplexvirus genomes, LHV-4 has a high overall G+C content of 65-70% in the unique regions and 75-77% in the inverted repeat regions. Orthologs of ICP34.5 and US5 were not identified in the LHV-4 genome. This study shows that LHV-4 has the smallest simplexvirus genome characterized to date.