Analysis of the genome of leporid herpesvirus 4.

TitleAnalysis of the genome of leporid herpesvirus 4.
Publication TypeJournal Article
Year of Publication2012
AuthorsBabra B, Watson G, Xu W, Jeffrey BM, Xu J-R, Rockey DD, Rohrmann GF, Jin L
Date Published2012 Nov 10
KeywordsAnimals, Base Composition, Cells, Cultured, DNA, Viral, Genome Size, Genome, Viral, High-Throughput Nucleotide Sequencing, Humans, Inverted Repeat Sequences, Molecular Sequence Data, Open Reading Frames, Phylogeny, Rabbits, Restriction Mapping, Sequence Analysis, DNA, Simplexvirus, Viral Proteins

The genome of a herpesvirus highly pathogenic to rabbits, leporid herpesvirus 4 (LHV-4), was analyzed using high-throughput DNA sequencing technology and primer walking. The assembled DNA sequences were further verified by restriction endonuclease digestion and Southern blot analyses. The total length of the LHV-4 genome was determined to be about 124 kb. Genes encoded in the LHV-4 genome are most closely related to herpesvirus of the Simplexvirus genus, including human herpesviruses (HHV-1 and HHV-2), monkey herpesviruses including cercopithicine (CeHV-2 and CeHV-16), macacine (McHV-1), bovine herpesvirus 2 (BHV-2), and a lineage of wallaby (macropodid) herpesviruses (MaHV-1 and -2). Similar to other simplexvirus genomes, LHV-4 has a high overall G+C content of 65-70% in the unique regions and 75-77% in the inverted repeat regions. Orthologs of ICP34.5 and US5 were not identified in the LHV-4 genome. This study shows that LHV-4 has the smallest simplexvirus genome characterized to date.

Alternate JournalVirology