Analysis of pyroptosis in bacterial infection.

TitleAnalysis of pyroptosis in bacterial infection.
Publication TypeJournal Article
Year of Publication2013
AuthorsDanelishvili L, Bermudez LE
JournalMethods in molecular biology (Clifton, N.J.)
Date Published2013
KeywordsApoptosis, Bacterial Infections, Caspase 1, Cell Line, Cytokines, Cytological Techniques, Enzyme Assays, Humans, Interleukin-18, Interleukin-1beta, Macrophages, Mycobacterium tuberculosis

Eukaryotic cells undergo death by several different mechanisms: apoptosis, a cell death that prevents inflammatory response; necrosis, when the cell membrane lyses and all the intracellular content is spilled outside; and pyroptosis, a cell death that is accompanied by the release of inflammatory cytokines by the dying cells. Pyroptosis is designed to attract a nonspecific innate response to the site of infection or tumor. In this chapter, we describe the methods used to study pyroptosis in a mammalian cell. The model organism used is Mycobacterium tuberculosis, which suppresses pyroptosis by macrophages, and possibly in dendritic cells.

Alternate JournalMethods Mol. Biol.