Analysis of pyroptosis in bacterial infection.

TitleAnalysis of pyroptosis in bacterial infection.
Publication TypeJournal Article
Year of Publication2013
AuthorsDanelishvili L, Bermudez LE
JournalMethods in molecular biology (Clifton, N.J.)
Volume1004
Pagination67-73
Date Published2013
ISSN1940-6029
KeywordsApoptosis, Bacterial Infections, Caspase 1, Cell Line, Cytokines, Cytological Techniques, Enzyme Assays, Humans, Interleukin-18, Interleukin-1beta, Macrophages, Mycobacterium tuberculosis
Abstract

Eukaryotic cells undergo death by several different mechanisms: apoptosis, a cell death that prevents inflammatory response; necrosis, when the cell membrane lyses and all the intracellular content is spilled outside; and pyroptosis, a cell death that is accompanied by the release of inflammatory cytokines by the dying cells. Pyroptosis is designed to attract a nonspecific innate response to the site of infection or tumor. In this chapter, we describe the methods used to study pyroptosis in a mammalian cell. The model organism used is Mycobacterium tuberculosis, which suppresses pyroptosis by macrophages, and possibly in dendritic cells.

DOI10.1007/978-1-62703-383-1_6
Alternate JournalMethods Mol. Biol.