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Baculovirus expression of the fusion protein gene of bovine respiratory syncytial virus and utility of the recombinant protein in a diagnostic enzyme immunoassay.
|Title||Baculovirus expression of the fusion protein gene of bovine respiratory syncytial virus and utility of the recombinant protein in a diagnostic enzyme immunoassay.|
|Publication Type||Journal Article|
|Year of Publication||1998|
|Authors||Pastey M, Samal SK|
|Journal||Journal of clinical microbiology|
|Date Published||1998 Apr|
|Keywords||Animals, Antibodies, Viral, Baculoviridae, Cattle, Enzyme-Linked Immunosorbent Assay, Humans, Immune Sera, Rabbits, Recombinant Proteins, Respiratory Syncytial Virus Infections, Respiratory Syncytial Virus, Bovine, Vaccination, Viral Fusion Proteins|
The fusion (F) protein of bovine respiratory syncytial virus (BRSV) was expressed by using a baculovirus vector. Antigenicity was tested by immunofluorescence analysis with F-specific monoclonal and polyclonal antibodies. Antibodies to recombinant F protein raised in a rabbit neutralized BRSV and human respiratory syncytial virus infectivity when tested in a plaque reduction assay. The recombinant F protein was evaluated as a source of antigen in an enzyme-linked immunosorbent assay (ELISA), and this ELISA was compared with the virus neutralization (VN) test for detecting BRSV antibodies in 10 consecutive serum samples from four calves vaccinated with a live modified BRSV vaccine and from two nonvaccinated control calves. The ELISA with the baculovirus-expressed F protein as an antigen compared favorably with the VN test and is a rapid, sensitive, and specific method for detecting serum antibodies to BRSV.
|Alternate Journal||J. Clin. Microbiol.|