Baculovirus expression of the fusion protein gene of bovine respiratory syncytial virus and utility of the recombinant protein in a diagnostic enzyme immunoassay.

TitleBaculovirus expression of the fusion protein gene of bovine respiratory syncytial virus and utility of the recombinant protein in a diagnostic enzyme immunoassay.
Publication TypeJournal Article
Year of Publication1998
AuthorsPastey M, Samal SK
JournalJournal of clinical microbiology
Volume36
Issue4
Pagination1105-8
Date Published1998 Apr
ISSN0095-1137
KeywordsAnimals, Antibodies, Viral, Baculoviridae, Cattle, Enzyme-Linked Immunosorbent Assay, Humans, Immune Sera, Rabbits, Recombinant Proteins, Respiratory Syncytial Virus Infections, Respiratory Syncytial Virus, Bovine, Vaccination, Viral Fusion Proteins
Abstract

The fusion (F) protein of bovine respiratory syncytial virus (BRSV) was expressed by using a baculovirus vector. Antigenicity was tested by immunofluorescence analysis with F-specific monoclonal and polyclonal antibodies. Antibodies to recombinant F protein raised in a rabbit neutralized BRSV and human respiratory syncytial virus infectivity when tested in a plaque reduction assay. The recombinant F protein was evaluated as a source of antigen in an enzyme-linked immunosorbent assay (ELISA), and this ELISA was compared with the virus neutralization (VN) test for detecting BRSV antibodies in 10 consecutive serum samples from four calves vaccinated with a live modified BRSV vaccine and from two nonvaccinated control calves. The ELISA with the baculovirus-expressed F protein as an antigen compared favorably with the VN test and is a rapid, sensitive, and specific method for detecting serum antibodies to BRSV.

Alternate JournalJ. Clin. Microbiol.