Cell penetrating peptide conjugates of steric block oligonucleotides.

TitleCell penetrating peptide conjugates of steric block oligonucleotides.
Publication TypeJournal Article
Year of Publication2008
AuthorsLebleu B, Moulton HM, Abes R, Ivanova GD, Abes S, Stein DA, Iversen PL, Arzumanov AA, Gait MJ
JournalAdvanced drug delivery reviews
Volume60
Issue4-5
Pagination517-29
Date Published2008 Mar 1
ISSN0169-409X
KeywordsAnimals, Cell Membrane, Cell Membrane Permeability, Drug Delivery Systems, Humans, Molecular Structure, Oligonucleotides, Peptide Nucleic Acids, Peptides
Abstract

Charge neutral steric block oligonucleotide analogues, such as peptide nucleic acids (PNA) or phosphorodiamidate morpholino oligomers (PMO), have promising biological and pharmacological properties for antisense applications, such as for example in mRNA splicing redirection. However, cellular uptake of free oligomers is poor and the utility of conjugates of PNA or PMO to cell penetrating peptides (CPP), such as Tat or Penetratin, is limited by endosomal sequestration. Two new families of arginine-rich CPPs named (R-Ahx-R)(4) AhxB and R(6)Pen allow efficient nuclear delivery of splice correcting PNA and PMO at micromolar concentrations in the absence of endosomolytic agents. The in vivo efficacy of (R-Ahx-R)(4) AhxB PMO conjugates has been demonstrated in mouse models of Duchenne muscular dystrophy and in various viral infections.

DOI10.1016/j.addr.2007.09.002
Alternate JournalAdv. Drug Deliv. Rev.