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Changes in molecular expression of aggrecan and collagen types I, II, and X, insulin-like growth factor-I, and transforming growth factor-beta1 in articular cartilage obtained from horses with naturally acquired osteochondrosis.
|Title||Changes in molecular expression of aggrecan and collagen types I, II, and X, insulin-like growth factor-I, and transforming growth factor-beta1 in articular cartilage obtained from horses with naturally acquired osteochondrosis.|
|Publication Type||Journal Article|
|Year of Publication||2001|
|Authors||Semevolos SA, Nixon AJ, Brower-Toland BD|
|Journal||American journal of veterinary research|
|Date Published||2001 Jul|
|Keywords||Aggrecans, Animals, Blotting, Northern, Cartilage, Articular, Collagen, Extracellular Matrix Proteins, Gene Expression Regulation, Developmental, Horse Diseases, Horses, Immunohistochemistry, In Situ Hybridization, Insulin-Like Growth Factor I, Joint Diseases, Lectins, C-Type, Osteochondritis, Proteoglycans, Reverse Transcriptase Polymerase Chain Reaction, RNA, Transforming Growth Factor beta, Transforming Growth Factor beta1|
OBJECTIVE: To determine molecular changes in the expression of insulin-like growth factor-I (IGF-I) and transforming growth factor-beta1 (TGF-beta1) in horses with osteochondrosis, and to characterize expression of matrix aggrecan and collagen types I, II, and X in articular cartilage of affected joints.
SAMPLE POPULATION: Articular cartilage from affected stifle or shoulder joints of 11 horses with naturally acquired osteochondrosis and corresponding joints of 11 clinically normal horses.
PROCEDURE: Harvested specimens were snap frozen in liquid nitrogen, and total RNA was isolated. Specimens were fixed in 4% paraformaldehyde for histologic examinations. Expression of matrix molecules was assessed by analysis of northern blots and in situ hybridization, using equine-specific cDNA probes and riboprobes, respectively. Expression of IGF-I and TGF-beta1 was assessed by use of noncompetitive quantitative polymerase chain reaction, in situ hybridization, and immunohistochemical analysis.
RESULTS: Cartilage obtained from osteochondrosis lesions had significantly greater expression of IGF-I, compared with normal cartilage. Expression of TGF-beta1 and collagen type I were higher, but not significantly so, in affected tissues. Expression of aggrecan or collagen types II and X did not differ between affected and clinically normal cartilage.
CONCLUSIONS AND CLINICAL RELEVANCE: Increased expression of growth factors and collagen type I was found in cartilage from osteochondrosis lesions. However, this probably reflects a healing response to injured tissue rather than a primary alteration. Therefore, methods aimed at altering concentrations of growth factors in cartilage of growing horses would be unlikely to alter the incidence or progress of the disease.
|Alternate Journal||Am. J. Vet. Res.|