Characterization and expression of secA in Mycobacterium avium.

TitleCharacterization and expression of secA in Mycobacterium avium.
Publication TypeJournal Article
Year of Publication2001
AuthorsLimia A, Sangari FJ, Wagner D, Bermudez LE
JournalFEMS microbiology letters
Volume197
Issue2
Pagination151-7
Date Published2001 Apr 13
ISSN0378-1097
KeywordsAdenosine Triphosphatases, Bacterial Proteins, Base Sequence, Biofilms, Carrier Proteins, Cloning, Molecular, Culture Media, Escherichia coli Proteins, Humans, Macrophages, Membrane Transport Proteins, Molecular Sequence Data, Mycobacterium avium, Promoter Regions, Genetic, Water
Abstract

Mycobacterium avium is both a pathogen that infects several hosts such as humans, pigs, and birds, as well as a microorganism that is encountered in environmental sources (soil and water). Protein secretion by the bacterium is likely to influence its ability to overcome adverse and competitive conditions both within or outside the host. Using a combination of cloning and information available in the databank, we characterized the secA gene from M. avium, encoding for a major preprotein translocase subunit associated with the secretion system of prokaryotics. In addition, we cloned the secA promoter sequence in a reporter construct upstream of a promoterless gfp. It was determined that the secA of M. avium shares large homology with the secA of Mycobacterium tuberculosis but not with secA of Mycobacterium leprae. secA expression was determined to be greater at logarithmic growth phase although it was also expressed at low levels during the stationary phase. secA expression was also observed when the bacteria were incubated in water as well as within human monocyte-derived macrophages and in conditions that are associated with biofilm formation. Future evaluation of the sec pathway in M. avium might provide important information about secreted proteins that are required for survival in different environments.

Alternate JournalFEMS Microbiol. Lett.