- Future Students
- Current Students
- Faculty & Staff
Characterization of arachidonic acid metabolism, superoxide production, and bacterial killing in bovine circulating neutrophils and elicited alveolar neutrophils.
|Title||Characterization of arachidonic acid metabolism, superoxide production, and bacterial killing in bovine circulating neutrophils and elicited alveolar neutrophils.|
|Publication Type||Journal Article|
|Year of Publication||1989|
|Authors||Heidel JR, Taylor SM, Laegreid WW, Silflow RM, Liggitt HD, Leid RW|
|Journal||Journal of leukocyte biology|
|Date Published||1989 Jul|
|Keywords||Animals, Arachidonic Acid, Arachidonic Acids, Blood Bactericidal Activity, Cattle, Haemophilus, Hydroxyeicosatetraenoic Acids, Leukotriene B4, Male, Neutrophils, Platelet Activating Factor, Pulmonary Alveoli, Staphylococcus epidermidis, Superoxides|
The in vitro generation and release of 5-lipoxygenase metabolites of arachidonic acid by bovine peripheral blood neutrophils and alveolar neutrophils elicited with either a heat-killed bacterium, Haemophilus somnus, or platelet-activating factor, were compared. After stimulation with calcium ionophore A23187 for 2.5-60 min, up to 4.5 +/- 0.7 (mean +/- SEM) ng of LTB4 per 10(6) cells was released into the media by circulating neutrophils. LTB4 release by alveolar neutrophils was significantly less (P less than .05) than that of peripheral blood neutrophils from the same animal; 5-HETE release by circulating neutrophils was maximal after 5 min stimulation by ionophore (1.2 +/- 0.1 ng/10(6) cells) but was not identified in cell culture media after 20 min. Alveolar neutrophils released similar amounts of 5-HETE when compared to circulating neutrophils, and release of 5-HETE by alveolar neutrophils was maximal after 5 min of stimulation. However, the 5-HETE released into the culture media persisted throughout the 60 min time period at levels which were maximal (1.5 +/- 0.2 to 1.8 +/- 0.3 ng/10(6) cells). Bacterial killing and the release of superoxide anion were not different between the two cell populations.
|Alternate Journal||J. Leukoc. Biol.|