- Future Students
- Current Students
- Faculty & Staff
Chlamydia trachomatis variant with nonfusing inclusions: growth dynamic and host-cell transcriptional response.
|Title||Chlamydia trachomatis variant with nonfusing inclusions: growth dynamic and host-cell transcriptional response.|
|Publication Type||Journal Article|
|Year of Publication||2005|
|Authors||Xia M, Suchland RJ, Bumgarner RE, Peng T, Rockey DD, Stamm WE|
|Journal||The Journal of infectious diseases|
|Date Published||2005 Oct 1|
|Keywords||Chlamydia trachomatis, Gene Expression Regulation, Genetic Variation, Hela Cells, Humans, Inclusion Bodies, Lipids, Membrane Fusion, Oligonucleotide Array Sequence Analysis, Proteins, Reverse Transcriptase Polymerase Chain Reaction, RNA, Ribosomal, 16S, Transcription, Genetic|
We compared growth rate and host-cell transcriptional responses of a Chlamydia trachomatis variant strain and a prototype strain. Growth dynamics were estimated by 16S rRNA level and by inclusion-forming units (IFUs) at different times after infection in HeLa cells. When inoculated at the same multiplicity of infection and observed 24-48 h after infection, the variant 16S rRNA transcriptional level was 3%-4% that of the prototype, and the IFUs of the variant strain were 0.1%-1% those of the prototype. Specific host-cell transcriptional responses to the variant were identified in a global-expression microarray in which variant strain-infected cells were compared with mock-infected and prototype strain-infected cells. In variant strain-infected cells, 47% (16/34) of specifically induced host genes were related to immunity and 32% (8/25) of specifically suppressed genes were related to lipid metabolism. The variant strain grew significantly more slowly and induced a modified host-cell transcriptional response, compared with the prototype strain.
|Alternate Journal||J. Infect. Dis.|