Chlamydia trachomatis variant with nonfusing inclusions: growth dynamic and host-cell transcriptional response.

TitleChlamydia trachomatis variant with nonfusing inclusions: growth dynamic and host-cell transcriptional response.
Publication TypeJournal Article
Year of Publication2005
AuthorsXia M, Suchland RJ, Bumgarner RE, Peng T, Rockey DD, Stamm WE
JournalThe Journal of infectious diseases
Volume192
Issue7
Pagination1229-36
Date Published2005 Oct 1
ISSN0022-1899
KeywordsChlamydia trachomatis, Gene Expression Regulation, Genetic Variation, Hela Cells, Humans, Inclusion Bodies, Lipids, Membrane Fusion, Oligonucleotide Array Sequence Analysis, Proteins, Reverse Transcriptase Polymerase Chain Reaction, RNA, Ribosomal, 16S, Transcription, Genetic
Abstract

We compared growth rate and host-cell transcriptional responses of a Chlamydia trachomatis variant strain and a prototype strain. Growth dynamics were estimated by 16S rRNA level and by inclusion-forming units (IFUs) at different times after infection in HeLa cells. When inoculated at the same multiplicity of infection and observed 24-48 h after infection, the variant 16S rRNA transcriptional level was 3%-4% that of the prototype, and the IFUs of the variant strain were 0.1%-1% those of the prototype. Specific host-cell transcriptional responses to the variant were identified in a global-expression microarray in which variant strain-infected cells were compared with mock-infected and prototype strain-infected cells. In variant strain-infected cells, 47% (16/34) of specifically induced host genes were related to immunity and 32% (8/25) of specifically suppressed genes were related to lipid metabolism. The variant strain grew significantly more slowly and induced a modified host-cell transcriptional response, compared with the prototype strain.

Alternate JournalJ. Infect. Dis.