Clostridium perfringens type E animal enteritis isolates with highly conserved, silent enterotoxin gene sequences.

TitleClostridium perfringens type E animal enteritis isolates with highly conserved, silent enterotoxin gene sequences.
Publication TypeJournal Article
Year of Publication1998
AuthorsBillington SJ, Wieckowski EU, Sarker MR, Bueschel D, Songer JG, McClane BA
JournalInfection and immunity
Volume66
Issue9
Pagination4531-6
Date Published1998 Sep
ISSN0019-9567
KeywordsAnimals, Base Sequence, Cattle, Cattle Diseases, Clostridium Infections, Clostridium perfringens, Conserved Sequence, DNA, Bacterial, Enteritis, Enterotoxins, Genes, Bacterial, Molecular Sequence Data
Abstract

Several Clostridium perfringens genotype E isolates, all associated with hemorrhagic enteritis of neonatal calves, were identified by multiplex PCR. These genotype E isolates were demonstrated to express alpha and iota toxins, but, despite carrying sequences for the gene (cpe) encoding C. perfringens enterotoxin (CPE), were unable to express CPE. These silent cpe sequences were shown to be highly conserved among type E isolates. However, relative to the functional cpe gene of type A isolates, these silent type E cpe sequences were found to contain nine nonsense and two frameshift mutations and to lack the initiation codon, promoters, and ribosome binding site. The type E animal enteritis isolates carrying these silent cpe sequences do not appear to be clonally related, and their silent type E cpe sequences are always located, near the iota toxin genes, on episomal DNA. These findings suggest that the highly conserved, silent cpe sequences present in most or all type E isolates may have resulted from the recent horizontal transfer of an episome, which also carries iota toxin genes, to several different type A C. perfringens isolates.

Alternate JournalInfect. Immun.