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Complementation of a Clostridium perfringens spo0A mutant with wild-type spo0A from other Clostridium species.
|Title||Complementation of a Clostridium perfringens spo0A mutant with wild-type spo0A from other Clostridium species.|
|Publication Type||Journal Article|
|Year of Publication||2006|
|Authors||Huang I-H, Sarker MR|
|Journal||Applied and environmental microbiology|
|Date Published||2006 Sep|
|Keywords||Amino Acid Sequence, Bacterial Proteins, Base Sequence, Clostridium, Clostridium perfringens, DNA Primers, DNA, Bacterial, Genes, Bacterial, Genetic Complementation Test, Molecular Sequence Data, Mutation, RNA, Bacterial, RNA, Messenger, Sequence Homology, Amino Acid, Species Specificity, Spores, Bacterial|
To evaluate whether C. perfringens can be used as a model organism for studying the sporulation process in other clostridia, C. perfringens spo0A mutant IH101 was complemented with wild-type spo0A from four different Clostridium species. Wild-type spo0A from C. acetobutylicum or C. tetani, but not from C. botulinum or C. difficile, restored sporulation and enterotoxin production in IH101. The ability of spo0A from C. botulinum or C. difficile to complement the lack of spore formation in IH101 might be due, at least in part, to the low levels of spo0A transcription and Spo0A production.
|Alternate Journal||Appl. Environ. Microbiol.|