Description of five new Loma (Microsporidia) species in pacific fishes with redesignation of the type species Loma morhua Morrison & Sprague, 1981, based on morphological and molecular species-boundaries tests.

TitleDescription of five new Loma (Microsporidia) species in pacific fishes with redesignation of the type species Loma morhua Morrison & Sprague, 1981, based on morphological and molecular species-boundaries tests.
Publication TypeJournal Article
Year of Publication2010
AuthorsBrown AMV, Kent ML, Adamson ML
JournalThe Journal of eukaryotic microbiology
Volume57
Issue6
Pagination529-53
Date Published2010 Nov-Dec
ISSN1550-7408
KeywordsAnimals, DNA, Fungal, DNA, Ribosomal, DNA, Ribosomal Spacer, Fish Diseases, Fishes, Loma, Microscopy, Molecular Sequence Data, Peptide Elongation Factor 1, Phylogeny, Sequence Analysis, DNA
Abstract

Five new species of Loma were described from five Pacific fishes using light-microscopic and ultrastructural features along with phylogenetic analysis of the gene sequences of ribosomal RNA (rRNA) and elongation factor 1-alpha. Morphological data revealed both qualitative and quantitative differences in developmental stages and timing, vesicles, xenoma features, and spore sizes with statistical support that differentiated Loma pacificodae n. sp. in Pacific cod, Loma wallae n. sp. in walleye pollock, Loma kenti n. sp. in Pacific tomcod, Loma lingcodae n. sp. in lingcod, and Loma richardi n. sp. in sablefish from each other and other species in the genus. Phylogenetic analyses combined with monophyly tests supported species designations, but with low resolution in two cases perhaps due to rRNA paralogs or recent speciation. Loma branchialis in haddock was shown to be separate from Loma morhua in Atlantic cod, thereby making L. morhua, and not L. branchialis, the type species. A species from brook trout was shown to be a separate species from Loma salmonae, not a variant strain selected in the laboratory. By comparison with gadid host phylogeny, these Loma species appear to have coevolved with their hosts, first colonizing the Pacific basin about 12 million years ago.

DOI10.1111/j.1550-7408.2010.00508.x
Alternate JournalJ. Eukaryot. Microbiol.