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Development of a 13C-glycocholic acid blood test to assess bacterial metabolic activity of the small intestine in canines.
|Title||Development of a 13C-glycocholic acid blood test to assess bacterial metabolic activity of the small intestine in canines.|
|Publication Type||Journal Article|
|Year of Publication||2005|
|Authors||Suchodolski JS, Ruaux CG, Steiner JM, Fetz K, Berghoff N, Williams DA|
|Journal||Canadian journal of veterinary research = Revue canadienne de recherche vétérinaire|
|Date Published||2005 Oct|
|Keywords||Administration, Oral, Analysis of Variance, Animals, Bacteria, Bacterial Infections, Biological Markers, Carbon Isotopes, Cholagogues and Choleretics, Dogs, Dose-Response Relationship, Drug, Female, Glycocholic Acid, Intestine, Small, Mass Spectrometry|
The objectives of this study were to establish optimal doses of 13C-glycocolic acid (GCA) for use in a GCA blood test as a marker for canine small intestinal bacterial metabolic activity. Four doses of GCA were administered orally to 8 healthy dogs. Blood samples were collected at various time points up to 480 min. The percent dose/min of 13C administered as GCA (PCD) and cumulative PCD (CUMPCD) were determined by fractional mass spectrometry. No dog showed any clinically obvious side effects. Doses of 1 and 2 mg/kg of bodyweight (BW) led to a significant increase in PCD and CUMPCD (P < 0.001). The mean CUMPCD was significantly higher for the 1 mg/kg BW dose compared with the 2 and 4 mg/kg BW doses (P < 0.05). Administration of 1 mg/kg BW of 13C-glycocholic acid led to an increase in CUMPCD over baseline in gas extracted from blood samples and appears to be the best parameter to evaluate for future clinical studies.
|Alternate Journal||Can. J. Vet. Res.|