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EDP-420, a bicyclolide (bridged bicyclic macrolide), is active against Mycobacterium avium.
|Title||EDP-420, a bicyclolide (bridged bicyclic macrolide), is active against Mycobacterium avium.|
|Publication Type||Journal Article|
|Year of Publication||2007|
|Authors||Bermudez LE, Motamedi N, Chee C, Baimukanova G, Kolonoski P, Inderlied C, Aralar P, Wang G, Phan LT, Young LS|
|Journal||Antimicrobial agents and chemotherapy|
|Date Published||2007 May|
|Keywords||Animals, Anti-Bacterial Agents, Bridged Compounds, Dose-Response Relationship, Drug, Drug Resistance, Bacterial, Female, Humans, Macrolides, Mice, Mice, Inbred C57BL, Microbial Sensitivity Tests, Mycobacterium avium|
Infection caused by Mycobacterium avium complex (MAC) is common in patients with immunosuppression, such as AIDS, and deficiencies of gamma interferon and interleukin-12, as well as patients with chronic lung diseases. Treatment of MAC disease is limited since few drugs show in vivo activity. We tested a new bridged bicyclic macrolide, EDP-420, against MAC in vitro and in beige mice. EDP-420 was inhibitory in vitro at a concentration ranging from 2 to 8 microg/ml (MIC(50) of 4 microg/ml and MIC(90) of 8 microg/ml). In macrophages, EDP-420 was inhibitory at 0.5 microg/ml, suggesting that the drug concentrates intracellularly. Mice infected with macrolide-susceptible MAC strain 101 were given 100 mg of EDP-420/kg of body weight daily for 4 weeks and showed a significant reduction in the number of bacteria in both liver and spleen which was greater than the reduction observed with clarithromycin treatment at the same dose (P < 0.05). However, macrolide-resistant MAC 101 did not respond to EDP-420 treatment. A combination of EDP-420 with mefloquine was shown to be indifferent; mefloquine alone was active against macrolide-resistant MAC. The frequency of resistance to EDP-420 in MAC 101 was 10(-9), which is significantly less than the emergence of resistance to clarithromycin, approximately 10(-7) (P < 0.05). Further evaluation of EDP-420 in the treatment of MAC disease is warranted.
|Alternate Journal||Antimicrob. Agents Chemother.|