Efficient inhibition of germination of coat-deficient bacterial spores by multivalent metal cations, including terbium (Tb³+).

TitleEfficient inhibition of germination of coat-deficient bacterial spores by multivalent metal cations, including terbium (Tb³+).
Publication TypeJournal Article
Year of Publication2011
AuthorsYi X, Bond C, Sarker MR, Setlow P
JournalApplied and environmental microbiology
Volume77
Issue15
Pagination5536-9
Date Published2011 Aug
ISSN1098-5336
KeywordsBacillus, Bacterial Proteins, Cations, Clostridium, Fluorescence, Microbial Viability, Picolinic Acids, Spores, Bacterial, Terbium
Abstract

Release of dipicolinic acid (DPA) and its fluorescence with terbium (Tb(3+)) allow rapid measurement of the germination and viability of spores of Bacillus and Clostridium species. However, germination of coat-deficient Bacillus spores was strongly inhibited by Tb(3+) and some other multivalent cations. Tb(3+) also inhibited germination of coat-deficient Clostridium perfringens spores.

Alternate JournalAppl. Environ. Microbiol.