Elemental analysis of the Mycobacterium avium phagosome in Balb/c mouse macrophages.

TitleElemental analysis of the Mycobacterium avium phagosome in Balb/c mouse macrophages.
Publication TypeJournal Article
Year of Publication2006
AuthorsWagner D, Maser J, Moric I, Vogt S, Kern WV, Bermudez LE
JournalBiochemical and biophysical research communications
Date Published2006 Jun 16
KeywordsAnimals, Calcium, Chlorine, Iron, Macrophages, Peritoneal, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mycobacterium avium, Phagosomes, Potassium, Tuberculosis, Zinc

Using a hard X-ray microprobe, we showed recently that in unstimulated peritoneal macrophages from C57BL/6 mice, the phagosome of pathogenic mycobacteria (Mycobacterium tuberculosis and Mycobacterium avium) can accumulate iron. We expanded our studies to the M. avium infection of peritoneal macrophages of Balb/c mice that show a similar degree of M. tuberculosis and M. avium-related chronic disease, but a higher susceptibility towards other intracellular pathogens such as Listeria monocytogenes, Leishmania major, or Brucella abortus as compared to C57BL/6 mice. Similar to C57BL/6 macrophages, the iron concentration in Balb/c macrophages increased significantly after 24 h of infection. A significant increase of the chlorine and potassium concentrations was observed in the Balb/c phagosomes between 1 and 24 h, in contrast with macrophages from C57BL/6 mice. The absolute elemental concentrations of calcium and zinc were higher in the mycobacterial phagosomes of Balb/c mice. We hypothesize that a potassium channel is abundant in the phagosome in macrophages that may be related to microbiocidal killing, similar to the requirement of potassium channels for microbiocidal function in neutrophils.

Alternate JournalBiochem. Biophys. Res. Commun.