Elemental analysis of the Mycobacterium avium phagosome in Balb/c mouse macrophages.

TitleElemental analysis of the Mycobacterium avium phagosome in Balb/c mouse macrophages.
Publication TypeJournal Article
Year of Publication2006
AuthorsWagner D, Maser J, Moric I, Vogt S, Kern WV, Bermudez LE
JournalBiochemical and biophysical research communications
Volume344
Issue4
Pagination1346-51
Date Published2006 Jun 16
ISSN0006-291X
KeywordsAnimals, Calcium, Chlorine, Iron, Macrophages, Peritoneal, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mycobacterium avium, Phagosomes, Potassium, Tuberculosis, Zinc
Abstract

Using a hard X-ray microprobe, we showed recently that in unstimulated peritoneal macrophages from C57BL/6 mice, the phagosome of pathogenic mycobacteria (Mycobacterium tuberculosis and Mycobacterium avium) can accumulate iron. We expanded our studies to the M. avium infection of peritoneal macrophages of Balb/c mice that show a similar degree of M. tuberculosis and M. avium-related chronic disease, but a higher susceptibility towards other intracellular pathogens such as Listeria monocytogenes, Leishmania major, or Brucella abortus as compared to C57BL/6 mice. Similar to C57BL/6 macrophages, the iron concentration in Balb/c macrophages increased significantly after 24 h of infection. A significant increase of the chlorine and potassium concentrations was observed in the Balb/c phagosomes between 1 and 24 h, in contrast with macrophages from C57BL/6 mice. The absolute elemental concentrations of calcium and zinc were higher in the mycobacterial phagosomes of Balb/c mice. We hypothesize that a potassium channel is abundant in the phagosome in macrophages that may be related to microbiocidal killing, similar to the requirement of potassium channels for microbiocidal function in neutrophils.

DOI10.1016/j.bbrc.2006.04.048
Alternate JournalBiochem. Biophys. Res. Commun.