<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Tenant, Rachel</style></author><author><style face="normal" font="default" size="100%">Bermudez, Luiz E</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Mycobacterium avium genes upregulated upon infection of Acanthamoeba castellanii demonstrate a common response to the intracellular environment.</style></title><secondary-title><style face="normal" font="default" size="100%">Current microbiology</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Curr. Microbiol.</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Acanthamoeba castellanii</style></keyword><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">Bacterial Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Gene Expression</style></keyword><keyword><style  face="normal" font="default" size="100%">Genes, Bacterial</style></keyword><keyword><style  face="normal" font="default" size="100%">Green Fluorescent Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Mycobacterium avium</style></keyword><keyword><style  face="normal" font="default" size="100%">Promoter Regions, Genetic</style></keyword><keyword><style  face="normal" font="default" size="100%">Up-Regulation</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2006</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2006 Feb</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">52</style></volume><pages><style face="normal" font="default" size="100%">128-33</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Using a previously created Mycobacterium avium genomic library with GFP-promoter trap in Mycobacterium smegmatis, we screened for genes that are upregulated upon infection of A. castellanii. Clones exhibiting a 2.5-fold or greater increase in GFP expression, out of a total of 10,000 clones, were selected for further examination. Upregulation was confirmed in subsequent experiments. A total of 20 clones showed an increase in expression 24 and 48 h after infection. Homologues were identified, and genes were found to encode for a variety of functions, including metabolic pathways, protein transcription and translation, and macromolecule degradation. Eight out of the 20 genes were found to be the same as those upregulated upon human macrophage infection. Five genes were selected to confirm upregulation in M. avium following amoeba infection, using real time PCR. All 5 genes were found to be upregulated at least 2.5-fold in M. avium. These results showed that the GFP promoter library in M. smegmatis is a valid system for studying gene upregulation in M. avium systems, and that many M. avium genes are commonly upregulated following macrophage and amoeba infection.</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><custom1><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/16450070?dopt=Abstract</style></custom1></record></records></xml>