<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Fosmire, Susan P</style></author><author><style face="normal" font="default" size="100%">Dickerson, Erin B</style></author><author><style face="normal" font="default" size="100%">Scott, Allyson M</style></author><author><style face="normal" font="default" size="100%">Bianco, Stacie R</style></author><author><style face="normal" font="default" size="100%">Pettengill, Marilyn J</style></author><author><style face="normal" font="default" size="100%">Meylemans, Heather</style></author><author><style face="normal" font="default" size="100%">Padilla, Marcia</style></author><author><style face="normal" font="default" size="100%">Frazer-Abel, Ashley A</style></author><author><style face="normal" font="default" size="100%">Akhtar, Nasim</style></author><author><style face="normal" font="default" size="100%">Getzy, David M</style></author><author><style face="normal" font="default" size="100%">Wojcieszyn, John</style></author><author><style face="normal" font="default" size="100%">Breen, Matthew</style></author><author><style face="normal" font="default" size="100%">Helfand, S C</style></author><author><style face="normal" font="default" size="100%">Modiano, Jaime F</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Canine malignant hemangiosarcoma as a model of primitive angiogenic endothelium.</style></title><secondary-title><style face="normal" font="default" size="100%">Laboratory investigation; a journal of technical methods and pathology</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Lab. Invest.</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">Antigens, CD</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Adhesion</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Division</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Line, Tumor</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Movement</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA, Neoplasm</style></keyword><keyword><style  face="normal" font="default" size="100%">Dog Diseases</style></keyword><keyword><style  face="normal" font="default" size="100%">Dogs</style></keyword><keyword><style  face="normal" font="default" size="100%">Endothelium, Vascular</style></keyword><keyword><style  face="normal" font="default" size="100%">Hemangiosarcoma</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Models, Biological</style></keyword><keyword><style  face="normal" font="default" size="100%">Neovascularization, Pathologic</style></keyword><keyword><style  face="normal" font="default" size="100%">Ploidies</style></keyword><keyword><style  face="normal" font="default" size="100%">Vascular Endothelial Growth Factor A</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2004</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2004 May</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">84</style></volume><pages><style face="normal" font="default" size="100%">562-72</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Hemangiosarcoma (HSA) is a common untreatable cancer of dogs that resembles human angiosarcoma. Detailed studies of these diseases have been historically hindered by the paucity of suitable reagents. Here, we show that expression of CD117 (c-Kit) can distinguish primitive (malignant) from mature (benign) proliferative endothelial lesions, and we describe eight independent cell lines derived from canine HSA explants. Endothelial origin was confirmed by sustained expression of surface CD105 (endoglin), CD146 (MUC18), and CD51/CD61 (alpha(v)beta(3) integrin). The cell lines showed anchorage-independent growth and were motile and invasive when cultured on a basement membrane matrix. They required endothelial growth factors for growth and survival, and they could be induced to form tubular structures resembling blood vessels when cultured under low calcium conditions. The formation of vessel-like structures was blocked by nicotine, and restored by FK506, suggesting that 'nuclear factor of activated T cells' activity prevents differentiation of these cells. In summary, these cell lines represent a unique and novel resource to improve our understanding of endothelial cell biology in general and canine HSA in particular.</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><custom1><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/15064773?dopt=Abstract</style></custom1></record></records></xml>