<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Craig, A M</style></author><author><style face="normal" font="default" size="100%">Blythe, L L</style></author><author><style face="normal" font="default" size="100%">Rowe, K E</style></author><author><style face="normal" font="default" size="100%">Lassen, E D</style></author><author><style face="normal" font="default" size="100%">Barrington, R</style></author><author><style face="normal" font="default" size="100%">Walker, K C</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Variability of alpha-tocopherol values associated with procurement, storage, and freezing of equine serum and plasma samples.</style></title><secondary-title><style face="normal" font="default" size="100%">American journal of veterinary research</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Am. J. Vet. Res.</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Analysis of Variance</style></keyword><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">Blood Preservation</style></keyword><keyword><style  face="normal" font="default" size="100%">Freezing</style></keyword><keyword><style  face="normal" font="default" size="100%">Horses</style></keyword><keyword><style  face="normal" font="default" size="100%">Plasma</style></keyword><keyword><style  face="normal" font="default" size="100%">Reproducibility of Results</style></keyword><keyword><style  face="normal" font="default" size="100%">Tissue and Organ Procurement</style></keyword><keyword><style  face="normal" font="default" size="100%">Vitamin E</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1992</style></year><pub-dates><date><style  face="normal" font="default" size="100%">1992 Dec</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">53</style></volume><pages><style face="normal" font="default" size="100%">2228-34</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Recent evidence concerning the pathogenesis of equine degenerative myeloencephalopathy indicated that low blood alpha-tocopherol values are a factor in the disease process. Variables that could be introduced by a veterinarian procuring, transporting, or storing samples were evaluated for effects on alpha-tocopherol concentration in equine blood. These variables included temperature; light; exposure to the rubber stopper of the evacuated blood collection tube; hemolysis; duration of freezing time, with and without nitrogen blanketing; and repeated freeze/thaw cycles. It was found that hemolysis caused the greatest change in high-performance liquid chromatography-measured serum alpha-tocopherol values, with mean decrease of 33% (P &lt; 0.001). Lesser, but significant (P &lt; 0.01) changes in serum alpha-tocopherol values were an approximate 10% decrease when refrigerated blood was left in contact with the red rubber stopper of the blood collection tube for 72 hours and an approximate 5% increase when blood was stored at 20 to 25 C (room temperature) for 72 hours. Repeated freeze/thaw cycles resulted in a significant (P &lt; 0.05) 3% decrease in alpha-tocopherol values in heparinized plasma by the third thawing cycle. Freezer storage for a 3-month period without nitrogen blanketing resulted in slight (2%) decrease in mean serum alpha-tocopherol values, whereas values in serum stored for an identical period under nitrogen blanketing did not change. A significant (P &lt; 0.001) mean decrease (10.3%) in alpha-tocopherol values was associated with freezer (-16 C) storage of nitrogen blanketed serum for 6 months.(ABSTRACT TRUNCATED AT 250 WORDS)</style></abstract><issue><style face="normal" font="default" size="100%">12</style></issue><custom1><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/1476303?dopt=Abstract</style></custom1></record></records></xml>