<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Johnson, Andy L</style></author><author><style face="normal" font="default" size="100%">Aravind, L</style></author><author><style face="normal" font="default" size="100%">Shulzhenko, Natalia</style></author><author><style face="normal" font="default" size="100%">Morgun, Andre</style></author><author><style face="normal" font="default" size="100%">Choi, See-Young</style></author><author><style face="normal" font="default" size="100%">Crockford, Tanya L</style></author><author><style face="normal" font="default" size="100%">Lambe, Teresa</style></author><author><style face="normal" font="default" size="100%">Domaschenz, Heather</style></author><author><style face="normal" font="default" size="100%">Kucharska, Edyta M</style></author><author><style face="normal" font="default" size="100%">Zheng, Lixin</style></author><author><style face="normal" font="default" size="100%">Vinuesa, Carola G</style></author><author><style face="normal" font="default" size="100%">Lenardo, Michael J</style></author><author><style face="normal" font="default" size="100%">Goodnow, Christopher C</style></author><author><style face="normal" font="default" size="100%">Cornall, Richard J</style></author><author><style face="normal" font="default" size="100%">Schwartz, Ronald H</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Themis is a member of a new metazoan gene family and is required for the completion of thymocyte positive selection.</style></title><secondary-title><style face="normal" font="default" size="100%">Nature immunology</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Nat. Immunol.</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Amino Acid Sequence</style></keyword><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">CD4-Positive T-Lymphocytes</style></keyword><keyword><style  face="normal" font="default" size="100%">CD8-Positive T-Lymphocytes</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Differentiation</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Line</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Lineage</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Survival</style></keyword><keyword><style  face="normal" font="default" size="100%">Ethylnitrosourea</style></keyword><keyword><style  face="normal" font="default" size="100%">Female</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Mice</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular Sequence Data</style></keyword><keyword><style  face="normal" font="default" size="100%">Mutation</style></keyword><keyword><style  face="normal" font="default" size="100%">Oligonucleotide Array Sequence Analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Receptors, Antigen, T-Cell</style></keyword><keyword><style  face="normal" font="default" size="100%">Signal Transduction</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2009</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2009 Aug</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">831-9</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">T cell antigen receptor (TCR) signaling in CD4(+)CD8(+) double-positive thymocytes determines cell survival and lineage commitment, but the genetic and molecular basis of this process is poorly defined. To address this issue, we used ethylnitrosourea mutagenesis to identify a previously unknown T lineage-specific gene, Themis, which is critical for the completion of positive selection. Themis contains a tandem repeat of a unique globular domain (called 'CABIT' here) that includes a cysteine motif that defines a family of five uncharacterized vertebrate proteins with orthologs in most animal species. Themis-deficient thymocytes showed no substantial impairment in early TCR signaling but did show altered expression of genes involved in the cell cycle and survival before and during positive selection. Our data suggest a unique function for Themis in sustaining positive selection.</style></abstract><issue><style face="normal" font="default" size="100%">8</style></issue><custom1><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/19597497?dopt=Abstract</style></custom1></record></records></xml>