- Future Students
- Current Students
- Faculty & Staff
Functional studies of bovine alveolar neutrophils elicited with recombinant bovine IL-1 beta.
|Title||Functional studies of bovine alveolar neutrophils elicited with recombinant bovine IL-1 beta.|
|Publication Type||Journal Article|
|Year of Publication||1990|
|Authors||Heidel JR, Sassenfeld HM, Maliszewdki CR, Silflow RM, Baker PE, Taylor SM, Leid RW|
|Journal||Journal of immunology (Baltimore, Md. : 1950)|
|Date Published||1990 Feb 1|
|Keywords||Animals, Arachidonate 5-Lipoxygenase, Arachidonic Acid, Arachidonic Acids, Blood Bactericidal Activity, Bronchoalveolar Lavage Fluid, Cattle, Dose-Response Relationship, Drug, Hydroxyeicosatetraenoic Acids, Interleukin-1, Leukotrienes, Neutrophils, Pulmonary Alveoli, Recombinant Proteins, Superoxides|
Bovine rIL-1 beta (rbIL-1 beta) was instilled intrabronchially into the lungs of steers to elicit harvestable alveolar neutrophils for functional analysis. Before instillation, bronchoalveolar lavage samples from the steers consisted of 96.4 +/- 1.5% (mean +/- SEM) macrophages, with the remaining cells neutrophils and occasional lymphocytes. Four hours after instillation of 1.0 and 10.0 nmol of IL-1, the lavage samples consisted of 96.3 +/- 0.8% and 91.0 +/- 5.7% neutrophils, respectively. Alveolar neutrophils elicited with rbIL-1 beta and challenged with the calcium ionophore, A23187, released similar amounts of leukotriene B4 (LTB4) and its nonenzymatic isomer LTB I, and significantly greater amounts of 5-hydroxyeicosatetraenoic acid and the nonenzymatic isomer LTB II, when compared with circulating neutrophils. The rbIL-1 beta did not, by itself, stimulate release of arachidonate metabolites from circulating neutrophils in quantities that were detectable by HPLC. Circulating neutrophils, preincubated with rbIL-1 beta and stimulated with A23187, released significantly greater amounts of 5-hydroxyeicosatetraenoic acid and total 5-lipoxygenase metabolites when compared with control cells not incubated with rbIL-1 beta. Incubation of circulating neutrophils with rbIL-1 beta and A23187 concurrently resulted in a significantly increased release of all 5-lipoxygenase metabolites of arachidonate. However, both the release of superoxide anion and bacterial killing by rbIL-1 beta-elicited bovine alveolar neutrophils did not differ from the values obtained for circulating neutrophils.
|Alternate Journal||J. Immunol.|