Growth within macrophages increases the efficiency of Mycobacterium avium in invading other macrophages by a complement receptor-independent pathway.

TitleGrowth within macrophages increases the efficiency of Mycobacterium avium in invading other macrophages by a complement receptor-independent pathway.
Publication TypeJournal Article
Year of Publication1997
AuthorsBermudez LE, Parker A, Goodman JR
JournalInfection and immunity
Volume65
Issue5
Pagination1916-25
Date Published1997 May
ISSN0019-9567
KeywordsAntibodies, Blocking, Antigens, CD29, Apoptosis, Catalase, Culture Media, Dextran Sulfate, DNA Fragmentation, Heparin, Humans, Macrophage-1 Antigen, Macrophages, Microscopy, Electron, Mycobacterium avium Complex, Mycobacterium avium-intracellulare Infection, Phagocytosis, Polysaccharides, Receptors, Transferrin, Tumor Necrosis Factor-alpha
Abstract

Infections caused by organisms of the Mycobacterium avium complex occur in approximately 50 to 60% of patients with AIDS. M. avium is an intracellular pathogen that survives and multiplies within mononuclear phagocytes. In this study, we investigated the uptake of M. avium grown within macrophages (intracellular growth M. avium [IG]) by a second macrophage compared with M. avium cultured in broth (extracellular growth M. avium [EG]). The results showed that IG was six- to eightfold more efficient than EG in entering macrophages. In addition, while an anti-CR3 antibody was able to inhibit approximately 60% of EG uptake by macrophages, it failed to inhibit the entry of IG. In contrast to EG, IG uptake into macrophages was significantly inhibited in the presence of anti-beta1-integrin and anti-transferrin receptor antibodies. Entry into macrophages by alternate receptors was associated with resistance to tumor necrosis factor alpha (TNF-alpha) stimulation. While stimulation with TNF-alpha resulted in inhibition of the growth of EG, it was not associated with inhibition of intracellular growth of IG. Investigation of the reason why M. avium is able to sense the changes in the intracellular environment triggering a change to the invasive phenotype suggests a direct relationship with macrophage apoptosis. These results suggest that intracellular growth is associated with novel mechanisms of M. avium uptake of macrophages and that those mechanisms appear to offer advantages to the bacteria in escaping the host defense.

Alternate JournalInfect. Immun.