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Immunohistochemical assay for detecting estrogen receptors in canine mammary tumors.
|Title||Immunohistochemical assay for detecting estrogen receptors in canine mammary tumors.|
|Publication Type||Journal Article|
|Year of Publication||1999|
|Authors||Graham JC, O'Keefe DA, Gelberg HB|
|Journal||American journal of veterinary research|
|Date Published||1999 May|
|Keywords||Animals, Cell Nucleus, Dog Diseases, Dogs, Female, Immunohistochemistry, Male, Mammary Neoplasms, Animal, Receptors, Estrogen|
OBJECTIVE: To determine the estrogen receptor (ER) content of canine mammary gland tumors by use of immunohistochemical (IHC) examination of formalin-fixed sections.
SAMPLE POPULATION: 21 mammary gland tumors from 20 adult dogs.
PROCEDURE: ER were detected in formalin-fixed tissues, using an avidin-biotin alkaline phosphatase IHC assay and were quantified on fresh-frozen tumor samples, using a modified dextran-coated charcoal (DCC) assay.
RESULTS: 7 of 21 tumors had visually detectable nuclear ER by use of IHC staining, whereas 8 of 21 tumors were positive for ER by use of the DCC assay. The ER-positive cells in 5 IHC-positive tumors were epithelial cells with histologic criteria of early malignancy. The remaining 2 ER-positive tumors detected by use of IHC had ER-positive mast cells within areas of connective tissue around the tumor.
CONCLUSIONS: Immunohistochemistry is an additional method for detection of ER in canine mammary tumors. The major advantage of this type of assay is that it may be performed on formalin-fixed tissues, and individual ER-positive cells may be identified. Discovery of ER-positive mast cells by use of IHC is of concern, particularly if the ER status of a tumor is based on DCC results alone.
CLINICAL RELEVANCE: Because most canine mammary tumors are fixed in formalin prior to histologic evaluation, an IHC assay that identifies ER-positive cells is desirable. Adjunctive antiestrogen therapy could be administered to dogs with ER-positive tumors.
|Alternate Journal||Am. J. Vet. Res.|