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Immunophysiological studies of interleukin-2 and canine lymphocytes.
|Title||Immunophysiological studies of interleukin-2 and canine lymphocytes.|
|Publication Type||Journal Article|
|Year of Publication||1992|
|Authors||Helfand SC, Modiano JF, Nowell PC|
|Journal||Veterinary immunology and immunopathology|
|Date Published||1992 Jun|
|Keywords||Animals, Dog Diseases, Dogs, Interleukin-2, Lymphocyte Activation, Mast-Cell Sarcoma, Receptors, Interleukin-2, Recombinant Proteins, T-Lymphocytes, Tumor Necrosis Factor-alpha|
Interleukin-2-dependent pathways of lymphocyte activation were investigated in canine peripheral blood lymphocytes (PBL) following stimulation with T-cell mitogens including phytohemagglutinin, phorbol ester (TPA), calcium ionophore (ionomycin), and human recombinant interleukin-2 (hrIL-2). The ability of the stimulated cells to produce interleukin-2 (IL-2) was determined using murine indicator cell lines. IL-2 receptor expression by mitogen-stimulated canine PBL was confirmed by the binding of hrIL-2 with high affinity, and with characteristics comparable to those of the human and murine IL-2 receptor. Examination of serum and PBL from two dogs that were treated with hrIL-2 and human recombinant tumor necrosis factor for systemic mast cell tumors showed that in one dog, IL-2 could be measured in the serum. Concurrently, the in vitro mitogenic response of this dog's PBL to hrIL-2 occurred earlier, possibly reflecting an increase in the relative number of IL-2-responsive cells within the PBL population.
|Alternate Journal||Vet. Immunol. Immunopathol.|