Immunostimulatory effects of human recombinant interleukin-12 on peripheral blood mononuclear cells from normal dogs.

TitleImmunostimulatory effects of human recombinant interleukin-12 on peripheral blood mononuclear cells from normal dogs.
Publication TypeJournal Article
Year of Publication1999
AuthorsPhillips BS, Padilla ML, Dickerson EB, Lindstrom MJ, Helfand SC
JournalVeterinary immunology and immunopathology
Volume70
Issue3-4
Pagination189-201
Date Published1999 Sep 20
ISSN0165-2427
KeywordsAnimals, Dogs, Dose-Response Relationship, Drug, Female, Humans, Interleukin-12, Leukocytes, Mononuclear, Lymphocyte Activation, Male, Recombinant Proteins, Tumor Cells, Cultured
Abstract

Interleukin-12 (IL-12) plays a pivotal role in regulating cellular immune responses involving autoimmunity, infectious disease, and cancer. Human recombinant (hr) IL-12 is being evaluated for therapy of human cancer. We investigated the potential of hrIL-12 to activate canine peripheral blood mononuclear cells (PBMC) using proliferation and cytotoxicity as readouts. Human rIL-12 caused increased proliferation of PBMC, and enhanced lysis of allogeneic canine tumor targets mediated by PBMC from normal dogs in vitro. In addition, antibody-dependent cellular cytotoxicity (ADCC) mediated by canine PBMC was enhanced by hrIL-12. These results indicate that hrIL-12 is recognized by canine immune cells, triggering a number of immune responses in canine PBMC, that may be important for immunotherapy of canine cancer. Information from this investigation provides impetus for evaluation of the effects of hrIL-12 on PBMC from tumor-bearing dogs and should be helpful in the development of hrIL-12 as an immune cell activator in vivo in the dog.

Alternate JournalVet. Immunol. Immunopathol.