Interleukin-2 gene polymorphism is associated with renal but not cardiac transplant outcome.

TitleInterleukin-2 gene polymorphism is associated with renal but not cardiac transplant outcome.
Publication TypeJournal Article
Year of Publication2003
AuthorsMorgun A, Shulzhenko N, Rampim GF, Medina JOP, Machado PGP, Diniz RVZ, Almeida DR, Gerbase-Delima M
JournalTransplantation proceedings
Volume35
Issue4
Pagination1344-5
Date Published2003 Jun
ISSN0041-1345
KeywordsAcute Disease, Creatinine, Genotype, Graft Survival, Heart Transplantation, Histocompatibility Testing, Humans, Interleukin-2, Kidney Transplantation, Phenotype, Polymorphism, Genetic, Polymorphism, Restriction Fragment Length, Polymorphism, Single Nucleotide, Time Factors, Treatment Outcome
Abstract

It was recently shown that IL-2 gene single nucleotide polymorphism (SNP) at position -330 (G-->T) is related to in vitro cytokine production levels, with the T/T and T/G genotypes being associated with low production and the G/G genotype associated with high production. The objective of this study was to investigate a possible influence of this polymorphism on renal and cardiac allograft outcomes. IL-2 SNP G-T (-330) was determined by PCR-RFLP in 67 recipients of heart allografts and in 63 recipients of renal grafts from HLA-haplo-identical, related donors. A higher frequency of the T/T genotype was observed in renal transplant patients who experienced at least one acute rejection episode during the first 3 months after transplantation than in those without rejection during this period (80% vs 49%, respectively, P <.05). Accordingly, the same genotype tended to be more frequent in renal recipients with a 6-month serum creatinine level above 1.5 mg/dL (median value for the whole group of kidney recipients) than in patients with lower creatinine levels (79% vs 45%, P <.08). Regarding cardiac transplant recipients, no associations were observed concerning acute rejection or graft survival. The finding of the association of T/T but not T/G genotype with acute kidney rejection was unexpected considering that both genotypes were shown to be associated with equal (low) IL-2 in vitro production. Further studies are necessary not only to dissect the nature of IL-2 T/T genotype association with kidney rejection, but also to explain why this genotype does not apparently influence cardiac allograft outcome.

Alternate JournalTransplant. Proc.