An intestinal xenograft model for Cryptosporidium parvum infection.

TitleAn intestinal xenograft model for Cryptosporidium parvum infection.
Publication TypeJournal Article
Year of Publication1994
AuthorsThulin JD, Kuhlenschmidt MS, Rolsma MD, Current WL, Gelberg HB
JournalInfection and immunity
Date Published1994 Jan
KeywordsAnimals, Cryptosporidiosis, Disease Models, Animal, Jejunum, Mice, Mice, Nude, Rabbits, Transplantation, Heterologous

Paired segments of near-term fetal rabbit small intestine were transplanted subcutaneously into athymic nude mice. At 5 weeks postsurgery, the xenografts were inoculated intraluminally with Cryptosporidium parvum sporozoites. Parasites rapidly and reliably infected the xenograft mucosal epithelium. Lesions typical of cryptosporidiosis were readily apparent by light microscopy and scanning and transmission electron microscopy. Xenografts are well suited to the study of the early events of C. parvum infection and are of potential value in the evaluation of anticryptosporidial chemotherapeutic agents.

Alternate JournalInfect. Immun.