Isoelectric focusing of soluble proteins in the characterization of species and isolates of Nematodirus (Nematoda: Trichostrongyloidea).

TitleIsoelectric focusing of soluble proteins in the characterization of species and isolates of Nematodirus (Nematoda: Trichostrongyloidea).
Publication TypeJournal Article
Year of Publication1997
AuthorsRickard LG, Hoberg EP, Mulrooney DM, Zimmerman GL
JournalThe Journal of parasitology
Volume83
Issue5
Pagination895-901
Date Published1997 Oct
ISSN0022-3395
KeywordsAnimals, Cattle, Cattle Diseases, Densitometry, Female, Helminth Proteins, Hydrogen-Ion Concentration, Isoelectric Focusing, Male, Sex Characteristics, Sheep, Sheep Diseases, Solubility, Species Specificity, Trichostrongyloidea, Trichostrongyloidiasis
Abstract

Isoelectric focusing was performed on extracts from Nematodirus spathiger, Nematodirus filicollis, Nematodirus helvetianus, and 3 geographic isolates of Nematodirus battus. Gender-specific differences were noted within species; however, the overall protein profile of each species and isolate was distinct and reproducible and allowed unequivocal differentiation. A coefficient of similarity (Sm) for males of each species and isolate was calculated, and a dendrogram, based on evaluation of Sm by the unweighted pair-group method with arithmetic means, was produced. Although cluster analysis of the 3 isolates of N. battus indicates the North American and Weybridge isolates are similar, interpretation of the relationships and thus the history of introduction based on these data is equivocal. Isoelectric focusing is a robust method for establishing identity and has great utility in diagnostics. However, in the absence of selective histochemical staining, interpretation of identity and homology for specific bands and banding patterns is problematic, thus limiting the utility of this method for phylogenetic inference.

Alternate JournalJ. Parasitol.