Lymphocyte blastogenic response to ovalbumin in a model for canine allergy.

TitleLymphocyte blastogenic response to ovalbumin in a model for canine allergy.
Publication TypeJournal Article
Year of Publication2009
AuthorsDévaud N, Hall JA, Gaschen F, Vallan C, Doherr MG, Williamson L, Peel JE, Burgener IA
JournalVeterinary journal (London, England : 1997)
Volume181
Issue2
Pagination178-86
Date Published2009 Aug
ISSN1090-0233
KeywordsAnimals, Cell Division, Dog Diseases, Dogs, Erythema, Hypersensitivity, Lymphocyte Activation, Lymphocytes, Ovalbumin, Reference Values
Abstract

Lymphocyte stimulation tests (LST) were performed in five dogs sensitised with ovalbumin (OVA) and seven healthy dogs. In addition, all five OVA-sensitised and two control dogs were tested after two in vivo provocations with OVA-containing eye drops. The isolated cells were suspended in culture media containing OVA and were cultured for up to 12 days. Proliferation was measured as reduction in 5,6-carboxylfluorescein diacetate succinimidyl ester (CFSE) intensity by flow cytometry on days 0, 3, 6, 9 and 12. A cell proliferation index (CPI) for each day and the area under the curve (AUC) of the CPI was calculated for each dog. All OVA-sensitised dogs demonstrated increased erythema after conjunctival OVA application. The presence of OVA-specific lymphocytes was demonstrated in 2/5 OVA-sensitised dogs before and 4/5 after in vivo provocation. Using the AUC, the difference between OVA-sensitised and control dogs was significant in all three LST before in vivo provocation (P<0.05) and borderline significant (P=0.053) in 2/3 LST after provocation. The most significant difference in CPI was observed after 9 days of culture (P=0.001). This pilot study indicates that the LST allows detection of rare antigen specific memory T-cells in dogs previously sensitised to, but not concurrently undergoing challenge by a specific antigen.

Alternate JournalVet. J.