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Lymphocyte blastogenic response to ovalbumin in a model for canine allergy.
|Title||Lymphocyte blastogenic response to ovalbumin in a model for canine allergy.|
|Publication Type||Journal Article|
|Year of Publication||2009|
|Authors||Dévaud N, Hall JA, Gaschen F, Vallan C, Doherr MG, Williamson L, Peel JE, Burgener IA|
|Journal||Veterinary journal (London, England : 1997)|
|Date Published||2009 Aug|
|Keywords||Animals, Cell Division, Dog Diseases, Dogs, Erythema, Hypersensitivity, Lymphocyte Activation, Lymphocytes, Ovalbumin, Reference Values|
Lymphocyte stimulation tests (LST) were performed in five dogs sensitised with ovalbumin (OVA) and seven healthy dogs. In addition, all five OVA-sensitised and two control dogs were tested after two in vivo provocations with OVA-containing eye drops. The isolated cells were suspended in culture media containing OVA and were cultured for up to 12 days. Proliferation was measured as reduction in 5,6-carboxylfluorescein diacetate succinimidyl ester (CFSE) intensity by flow cytometry on days 0, 3, 6, 9 and 12. A cell proliferation index (CPI) for each day and the area under the curve (AUC) of the CPI was calculated for each dog. All OVA-sensitised dogs demonstrated increased erythema after conjunctival OVA application. The presence of OVA-specific lymphocytes was demonstrated in 2/5 OVA-sensitised dogs before and 4/5 after in vivo provocation. Using the AUC, the difference between OVA-sensitised and control dogs was significant in all three LST before in vivo provocation (P<0.05) and borderline significant (P=0.053) in 2/3 LST after provocation. The most significant difference in CPI was observed after 9 days of culture (P=0.001). This pilot study indicates that the LST allows detection of rare antigen specific memory T-cells in dogs previously sensitised to, but not concurrently undergoing challenge by a specific antigen.
|Alternate Journal||Vet. J.|