Measurements of plasma endothelin immunoreactivity in healthy cats and cats with cardiomyopathy.

TitleMeasurements of plasma endothelin immunoreactivity in healthy cats and cats with cardiomyopathy.
Publication TypeJournal Article
Year of Publication2004
AuthorsProsek R, Sisson DD, Oyama MA, Biondo AW, Solter PE
JournalJournal of veterinary internal medicine / American College of Veterinary Internal Medicine
Volume18
Issue6
Pagination826-30
Date Published2004 Nov-Dec
ISSN0891-6640
KeywordsAnimals, Biological Markers, Cardiomyopathies, Case-Control Studies, Cat Diseases, Cats, Echocardiography, Endothelin-1, Enzyme-Linked Immunosorbent Assay, Female, Male
Abstract

Plasma concentrations of endothelin-1 (ET-1), the most potent endogenous pressor substance discovered to date, are abnormally high in humans with congestive heart failure (CHF), and they correlate with the degree of functional impairment. We sought first to validate a human sandwich ELISA kit that targets that portion of the amino acid sequence that is identical in cats. The assay demonstrated linearity (R2 = .9968) and parallelism (P = .5339), recovery of spiked human ET-1 in cat plasma averaged 98.7%, and intraassay precision had a coefficient of variation <10%. We subsequently determined ET-1 immunoreactivity in healthy cats and in cats with myocardial disease with and without CHF, systemic thromboembolism (STE), or both. Plasma ET-1 immunoreactivity was measured in 12 healthy cats and in 28 cats with primary myocardial disease, including hypertrophic cardiomyopathy (HCM), dilated cardiomyopathy (DCM), or restrictive or unclassified cardiomyopathy (RCM and UCM), respectively. Plasma ET mean (95% CI) concentrations were 0.777 (0.6536-0.924) fmol/mL in the control cats, 1.427 (0.922-2.209) fmol/mL in 12 cats with cardiomyopathy (HCM = 11, RCM/UCM = 1) but without CHF or evidence of STE, and 2.360 (1.666-3.343) fmol/mL in 16 cats with cardiomyopathy (HCM = 8, RCM/UCM = 7, DCM = 1) and CHF (n = 15) or STE (n = 4). Plasma immunoreactivity of ET-1 was significantly higher in cats with myocardial disease without CHF/STE versus normal cats (P < .05) and in cats with myocardial disease with CHF/STE versus normal cats (P < .001).

Alternate JournalJ. Vet. Intern. Med.