Molecular and morphological analysis of Myxobolus spp. of salmonid fishes with the description of a new Myxobolus species.

TitleMolecular and morphological analysis of Myxobolus spp. of salmonid fishes with the description of a new Myxobolus species.
Publication TypeJournal Article
Year of Publication2008
AuthorsFerguson JA, Atkinson SD, Whipps CM, Kent ML
JournalThe Journal of parasitology
Volume94
Issue6
Pagination1322-34
Date Published2008 Dec
ISSN0022-3395
KeywordsAnimals, Cnidaria, DNA, Ribosomal, Fish Diseases, Muscles, Muscular Diseases, Parasitic Diseases, Animal, Peripheral Nervous System Diseases, Salmonidae
Abstract

While investigating the parasite fauna of wild coho salmon. Oncorhynchus kisutch (Walbaum, 1792), histological examination provided evidence of a new species of Myxobolus (Myxozoa: Myxosporea) infecting nerves of skeletal muscle. Spores were morphologically similar to those of the intramuscular Myxobolus insidiosus Wyatt and Pratt, 1963, both having pyriform spores with clavate polar capsules. However, the former developed exclusively in the nerves of skeletal muscle rather than in myocytes. We examined both species of Myxobolus derived from coho salmon; Chinook salmon, Oncorhynchus tshawytscha (Walbaum, 1792); cutthroat trout, Oncorhynchus clarkii (Richardson, 1836); and rainbow trout Oncorhynchus mykiss (Walbaum, 1792) from freshwater in Oregon. Spore morphology, small subunit ribosomal RNA gene (rDNA) sequences, and site of infection were compared. Myxobolus arcticus Pugachev and Khokhlov, 1979 has pyriform spores, infects the central nervous system of many salmonids, and is found in the Pacific Northwest. It was therefore included in the analyses to rule out conspecificity with the new species. Together, these data show that the Myxobolus sp. from peripheral nerves in the skeletal musculature of coho salmon, rainbow trout, and cutthroat trout is a new species, described herein as Myxobolus fryeri n. sp.

DOI10.1645/GE-1606.1
Alternate JournalJ. Parasitol.