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Mycobacterium avium grown in Acanthamoeba castellanii is protected from the effects of antimicrobials.
|Title||Mycobacterium avium grown in Acanthamoeba castellanii is protected from the effects of antimicrobials.|
|Publication Type||Journal Article|
|Year of Publication||2000|
|Authors||Miltner EC, Bermudez LE|
|Journal||Antimicrobial agents and chemotherapy|
|Date Published||2000 Jul|
|Keywords||Acanthamoeba, Animals, Anti-Bacterial Agents, Azithromycin, Clarithromycin, HT29 Cells, Humans, Mice, Microbial Sensitivity Tests, Mycobacterium avium, Rifabutin, U937 Cells|
Mycobacterium avium is a common cause of systemic bacterial infection in patients with AIDS. Infection with M. avium has been linked to bacterial colonization of domestic water supplies and commonly occurs through the gastrointestinal tract. Acanthamoeba castellanii, a waterborne protozoan, may serve as an environmental host for M. avium. It has been shown that growth of M. avium in amoebae enhances invasion and intracellular replication of the bacteria in human macrophages and intestinal epithelial cell line HT-29 as well as in mice. We determined that growth of M. avium within A. castellanii influenced susceptibility to rifabutin, azithromycin, and clarithromycin. No significant activity against M. avium was seen with rifabutin, azithromycin, and clarithromycin when used to treat monolayers on both day 1 and day 4 after infection. When tested in a macrophage-like cell line (U937), all compounds showed significant anti-M. avium activity. Growth of M. avium in amoebae appears to reduce the effectiveness of the antimicrobials. These findings may have significant implications for prophylaxis of M. avium infection in AIDS.
|Alternate Journal||Antimicrob. Agents Chemother.|