NhaP1 is a K+(Na+)/H+ antiporter required for growth and internal pH homeostasis of Vibrio cholerae at low extracellular pH.

TitleNhaP1 is a K+(Na+)/H+ antiporter required for growth and internal pH homeostasis of Vibrio cholerae at low extracellular pH.
Publication TypeJournal Article
Year of Publication2012
AuthorsQuinn MJ, Resch CT, Sun J, Lind EJ, Dibrov P, Häse CC
JournalMicrobiology (Reading, England)
Volume158
IssuePt 4
Pagination1094-105
Date Published2012 Apr
ISSN1465-2080
KeywordsBacterial Proteins, Cloning, Molecular, Cytoplasm, Gene Deletion, Homeostasis, Hydrogen-Ion Concentration, Potassium, Potassium-Hydrogen Antiporters, Sodium-Hydrogen Antiporter, Vibrio cholerae
Abstract

Vibrio cholerae has adapted to a wide range of salinity, pH and osmotic conditions, enabling it to survive passage through the host and persist in the environment. Among the many proteins responsible for bacterial survival under these diverse conditions, we have identified Vc-NhaP1 as a K(+)(Na(+))/H(+) antiporter essential for V. cholerae growth at low environmental pH. Deletion of the V. cholerae nhaP1 gene caused growth inhibition when external potassium was either limited (100 mM and below) or in excess (400 mM and above). This growth defect was most apparent at mid-exponential phase, after 4-6 h of culture. Using a pH-sensitive GFP, cytosolic pH was shown to be dependent on K(+) in acidic external conditions in a Vc-NhaP1-dependent manner. When functionally expressed in an antiporterless Escherichia coli strain and assayed in everted membrane vesicles, Vc-NhaP1 operated as an electroneutral alkali cation/proton antiporter, exchanging K(+) or Na(+) ions for H(+) within a broad pH range (7.25-9.0). These data establish the putative V. cholerae NhaP1 protein as a functional K(+)(Na(+))/H(+) antiporter of the CPA1 family that is required for bacterial pH homeostasis and growth in an acidic environment.

Alternate JournalMicrobiology (Reading, Engl.)