Phagocytosis and intracellular killing of Mycobacterium avium complex by human and murine macrophages.

TitlePhagocytosis and intracellular killing of Mycobacterium avium complex by human and murine macrophages.
Publication TypeJournal Article
Year of Publication1987
AuthorsBermudez LE, Young LS
JournalBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.]
Volume20
Issue2
Pagination191-201
Date Published1987
ISSN0100-879X
KeywordsAnimals, Macrophage Activation, Macrophages, Mice, Mycobacterium avium, Phagocytosis, Virulence
Abstract

1. Host defenses against Mycobacterium avium complex (MAC) are poorly defined. Peritoneal macrophages from black and beige mice, and cultured human macrophages were infected in vitro with MAC serotype 1 from an AIDS patient, in the presence or absence of normal or convalescent serum. Bacteria:cell ratio was 1:10. Supernatants and macrophage lysates were cultured 6, 24 and 48 h later to determine the uptake and killing by macrophages. Phagocytosis by activated macrophages, obtained from pre-infected and treated mice or stimulated in vitro with endotoxin, was also studied. 2. Neither convalescent serum nor normal serum caused a significant increase in MAC phagocytosis. 3. Unstimulated macrophages from black or beige mice and humans were incapable of killing the intracellular bacteria. Activated macrophages from all sources phagocytized and killed 80 +/- 4% of the initial inoculum after 48 h in culture. 4. These results demonstrate that activated macrophages are required for optimal intracellular killing of serotype 1 MAC.

Alternate JournalBraz. J. Med. Biol. Res.