Plasma endothelin-1 immunoreactivity in normal dogs and dogs with acquired heart disease.

TitlePlasma endothelin-1 immunoreactivity in normal dogs and dogs with acquired heart disease.
Publication TypeJournal Article
Year of Publication2004
AuthorsProsek R, Sisson DD, Oyama MA, Biondo AW, Solter PF
JournalJournal of veterinary internal medicine / American College of Veterinary Internal Medicine
Volume18
Issue6
Pagination840-4
Date Published2004 Nov-Dec
ISSN0891-6640
KeywordsAnimals, Biological Markers, Cardiomyopathies, Case-Control Studies, Dog Diseases, Dogs, Endothelin-1, Enzyme-Linked Immunosorbent Assay, Female, Heart Diseases, Heart Failure, Heart Valve Diseases, Male, Predictive Value of Tests
Abstract

We sought to measure plasma endothelin-1 (ET-1) concentrations in normal dogs and to compare them with those measured in dogs with acquired heart disease with or without pulmonary edema. A sandwich enzyme-linked immunosorbent assay kit was validated and used to measure ET-1 immunoreactivity in plasma samples obtained from 32 normal dogs and 46 dogs with either dilated cardiomyopathy (DCM, n = 27) or degenerative valvular disease (CDVD, n = 19) with (n = 30) or without (n = 16) overt congestive heart failure (CHF). Plasma ET-1 concentrations (geometric mean, 95% confidence interval of geometric mean) were 1.17 (1.04-1.32) fmol/mL in the 32 normal control dogs, 1.25 (0.981-1.60) fmol/mL in 16 dogs with DCM (n = 9) or CDVD (n = 7) without CHF, and 2.51 (2.10-3.01) fmol/mL in 30 dogs with DCM (n = 18) and CDVD (n = 12) with CHE Plasma immunoreactivity of ET-1 was significantly higher in dogs with CHF in comparison with normal dogs (P < .001) and dogs with heart disease without CHF (P < .001). No significant difference was found between normal dogs and dogs with heart disease but without CHF (P > .05). Significant correlations were between plasma ET-I concentrations and left atrial:aortic ratio (P < .0001, r2 = .39), left ventricular internal dimension at end-diastole indexed to aortic diameter (P < .0001, r2 = .30) or body surface area (BSA) (P = .0071, r2 = .10), and left ventricular internal dimension at end-systole indexed to aortic diameter (P = .0003, r- = .17) or BSA (P = .0008, r2 = .15).

Alternate JournalJ. Vet. Intern. Med.