A RhoA-derived peptide inhibits human immunodeficiency virus-1 entry in vitro.

TitleA RhoA-derived peptide inhibits human immunodeficiency virus-1 entry in vitro.
Publication TypeJournal Article
Year of Publication2011
AuthorsMaselko M, Ward C, Pastey M
JournalCurrent HIV research
Volume9
Issue1
Pagination1-5
Date Published2011 Jan 1
ISSN1873-4251
KeywordsCell Line, Cell Proliferation, Cytopathogenic Effect, Viral, HIV Envelope Protein gp41, HIV Fusion Inhibitors, HIV-1, Humans, Microbial Sensitivity Tests, Oligopeptides, Peptide Fragments, Protein Multimerization, rhoA GTP-Binding Protein, Virus Internalization
Abstract

RhoA-derived peptides have been shown to have antiviral activity against both human respiratory syncytial virus and human parainfluenza virus-3. The present study investigates the toxicity, anti-HIV-1 activity and mechanism of action of a RhoA-derived peptide (RhoA 77-95). The efficacy of this peptide was compared to a scrambled peptide of the same amino acid composition and Enfuvirtide, a HIV entry inhibitor. Our data show that this RhoA-derived peptide is a non-toxic and effective inhibitor of a CXCR4 tropic strain of HIV-1. We also demonstrate that the mechanism of entry inhibition is likely mediated by polyanionic properties and is dependent on the dimerization of peptides.

DOI10.1111/j.1365-3164.2011.00980.x
Alternate JournalCurr. HIV Res.