Role of sodium bioenergetics in Vibrio cholerae.

TitleRole of sodium bioenergetics in Vibrio cholerae.
Publication TypeJournal Article
Year of Publication2001
AuthorsHäse CC, Barquera B
JournalBiochimica et biophysica acta
Volume1505
Issue1
Pagination169-78
Date Published2001 May 1
ISSN0006-3002
KeywordsAmino Acid Sequence, Bacterial Proteins, Cations, Monovalent, Cell Membrane, Cholera, Energy Metabolism, Humans, Intracellular Membranes, Membrane Potentials, Models, Chemical, Proton-Translocating ATPases, Quinone Reductases, Sodium, Vibrio cholerae, Virulence, Water Microbiology
Abstract

The ability of the bacterium to use sodium in bioenergetic processes appears to play a key role in both the environmental and pathogenic phases of Vibrio cholerae. Aquatic environments, including fresh, brackish, and coastal waters, are an important factor in the transmission of cholera and an autochthonous source. The organism is considered to be halophilic and has a strict requirement for Na(+) for growth. Furthermore, expression of motility and virulence factors of V. cholerae is intimately linked to sodium bioenergetics and to each other. Several lines of evidence indicated that the activity of the flagellum of V. cholerae might have an impact on virulence gene regulation. As the V. cholerae flagellum is sodium-driven and the Na(+)-NQR enzyme is known to create a sodium motive force across the bacterial membrane, it was recently suggested that the increased toxT expression observed in a nqr-negative strain is mediated by affecting flagella activity. It was suggested that the V. cholerae flagellum might respond to changes in membrane potential and the resulting changes in flagellar rotation might serve as a signal for virulence gene expression. However, we recently demonstrated that although the flagellum of V. cholerae is not required for the effects of ionophores on virulence gene expression, changes in the sodium chemical potential are sensed and thus alternative mechanisms, perhaps involving the TcpP/H proteins, for the detection of these conditions must exist. Analyzing the underlying mechanisms by which bacteria respond to changes in the environment, such as their ability to monitor the level of membrane potential, will probably reveal complex interplays between basic physiological processes and virulence factor expression in a variety of pathogenic species.

Alternate JournalBiochim. Biophys. Acta