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Tetracycline resistance in Chlamydia suis mediated by genomic islands inserted into the chlamydial inv-like gene.
|Title||Tetracycline resistance in Chlamydia suis mediated by genomic islands inserted into the chlamydial inv-like gene.|
|Publication Type||Journal Article|
|Year of Publication||2004|
|Authors||Dugan J, Rockey DD, Jones L, Andersen AA|
|Journal||Antimicrobial agents and chemotherapy|
|Date Published||2004 Oct|
|Keywords||Animals, Bacterial Proteins, Blotting, Southern, Cercopithecus aethiops, Chlamydia, Chromosome Mapping, Cloning, Molecular, DNA Transposable Elements, Genes, Bacterial, Genome, Bacterial, Gram-Negative Bacteria, Microbial Sensitivity Tests, Plasmids, Repressor Proteins, Reverse Transcriptase Polymerase Chain Reaction, Swine, Tetracycline Resistance, Vero Cells|
Many strains of Chlamydia suis, a pathogen of pigs, express a stable tetracycline resistance phenotype. We demonstrate that this resistance pattern is associated with a resistance gene, tet(C), in the chlamydial chromosome. Four related genomic islands were identified in seven tetracycline-resistant C. suis strains. All resistant isolates carry the structural gene tet(C) and the tetracycline repressor gene tetR(C). The islands share significant nucleotide sequence identity with resistance plasmids carried by a variety of different bacterial species. Three of the four tet(C) islands also carry a novel insertion sequence that is homologous to the IS605 family of insertion sequences. In each strain, the resistance gene and associated sequences are recombined into an identical position in a gene homologous to the inv gene of the yersiniae. These genomic islands represent the first examples of horizontally acquired DNA integrated into a natural isolate of chlamydiae or within any other obligate intracellular bacterium.
|Alternate Journal||Antimicrob. Agents Chemother.|