Therapeutic effects of paclitaxel-containing ultrasound contrast agents.

TitleTherapeutic effects of paclitaxel-containing ultrasound contrast agents.
Publication TypeJournal Article
Year of Publication2006
AuthorsTartis MS, McCallan J, Lum AFH, LaBell R, Stieger SM, Matsunaga TO, Ferrara KW
JournalUltrasound in medicine & biology
Volume32
Issue11
Pagination1771-80
Date Published2006 Nov
ISSN0301-5629
KeywordsAnimals, Antineoplastic Agents, Phytogenic, Cell Death, Chick Embryo, Chorioallantoic Membrane, Contrast Media, Drug Carriers, Drug Delivery Systems, Humans, Integrin alphaVbeta3, Ligands, Melanoma, Microbubbles, Neoplasm Proteins, Paclitaxel, Sonication, Tumor Cells, Cultured
Abstract

Drug delivery vehicles that combine ultrasonic and molecular targeting are shown to locally concentrate a drug in a region-of-interest. The drug delivery vehicles, referred to as acoustically active lipospheres (AALs), are microbubbles surrounded by a shell of oil and lipid. In a region limited to the focal area of ultrasound application, circulating AALs are deflected by radiation force to a vessel wall and can subsequently be fragmented. Ligands targeting the alphavbeta3 integrin are conjugated to the AAL shell and increase in vitro binding by 26.5-fold over nontargeted agents. Toxicity assays demonstrate that paclitaxel-containing AALs exert a greater antiproliferative effect after insonation than free paclitaxel at an equivalent concentration. Lastly, ultrasound and molecular targeting are combined to deliver a model drug to the endothelium and interstitium of chorioallantoic membrane vasculature in vivo.

Alternate JournalUltrasound Med Biol