Tumor necrosis factor-alpha at presentation in 60 cases of spontaneous canine acute pancreatitis.

TitleTumor necrosis factor-alpha at presentation in 60 cases of spontaneous canine acute pancreatitis.
Publication TypeJournal Article
Year of Publication1999
AuthorsRuaux CG, Pennington HL, Worrall S, Atwell RB
JournalVeterinary immunology and immunopathology
Volume72
Issue3-4
Pagination369-76
Date Published1999 Dec 30
ISSN0165-2427
KeywordsAcute Disease, Animals, Dog Diseases, Dogs, Enzyme-Linked Immunosorbent Assay, Pancreatitis, Tumor Necrosis Factor-alpha
Abstract

Tumor necrosis factor-alpha (TNF) is a pleiotropic cytokine with profound and broad ranging effects on many cell types. There have been few publications investigating the role of TNF in spontaneous disease processes of dogs, particularly the role of this cytokine during endotoxaemia, shock and multiple organ dysfunction syndromes. Plasma samples taken at presentation from 60 dogs with spontaneous acute pancreatitis of varying severity levels (scored 0-4 in ascending severity) were assessed for TNF activity by bioassay and total TNF protein levels through a dot-blot immunoassay. TNF activity by bioassay was detected in 31% (4/13) of dogs presenting with severe disease (>50% expected mortality) as defined using a scoring system for organ compromise, and was not detectable in the remaining animals or healthy controls. TNF activity was detected in 66% (4/6) animals in the highest severity group (Score 4), these animals were showing severe multiple organ dysfunction. Total TNF protein levels, measured by dot-blot immunoassay, exhibited a wide range in all severity groups and healthy dogs. Dogs with detectable TNF activity were not distinguished from the other severity or healthy groups by immunoassay. The absence of detectable differences in total TNF protein levels between the various severity groups suggests that other factors may be crucial in determining the role of TNF in spontaneous canine acute pancreatitis and subsequent endotoxaemia and shock.

Alternate JournalVet. Immunol. Immunopathol.