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Ubiquitin expression in muscle from horses with polysaccharide storage myopathy.
|Title||Ubiquitin expression in muscle from horses with polysaccharide storage myopathy.|
|Publication Type||Journal Article|
|Year of Publication||2006|
|Authors||Valentine BA, Flint TH, Fischer KA|
|Date Published||2006 May|
|Keywords||Animals, Gene Expression Regulation, Glycogen Storage Disease, Horse Diseases, Horses, Muscle, Skeletal, Muscular Diseases, Polysaccharides, Ubiquitins|
Serial sections of formalin-fixed, paraffin-embedded muscle biopsy specimens from 28 Quarter Horse, Paint, and draft-related breeds, aged 0.5-23 years, were treated with periodic acid-Schiff (PAS) stain for glycogen and were immunostained to detect ubiquitin expression. On the basis of findings in PAS-stained sections, a diagnosis of equine polysaccharide storage myopathy (EPSSM) was made in 22 horses aged 2-23 years (mean, 9.4 years); samples from 6 horses aged 0.5-15 years (mean, 7.3 years) had a normal PAS staining pattern, with no relevant lesions. Ubiquitin expression was detected in all but a 2-year-old EPSSM-affected horse and was not detected in the non-EPSSM-affected horses. Ubiquitin expression was greater than the degree of PAS-positive, amylase-resistant material, and ubiquitin was detected in aggregates of amylase-sensitive glycogen as well as in aggregates of amylase-resistant material. Results suggest that glycogen aggregates develop and are ubiquitinated prior to development of amylase-resistant inclusions. Ubiquitin immunostaining may be most useful for confirming the diagnosis of EPSSM in horses with only amylase-sensitive glycogen aggregates and in horses with early amylase-resistant inclusions. However, ubiquitin immunostaining is no more sensitive than is PAS staining for diagnosis of EPSSM.
|Alternate Journal||Vet. Pathol.|